ST14A cells have properties of a medium-size spiny neuron
ST14A cells have properties of a medium-size spiny neuron
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DOI：
10.1006/exnr.2000.7551
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发表时间：
2001-02-01
影响因子：
5.3
通讯作者：
Cattaneo, E
中科院分区：
文献类型：
--
作者：
Ehrlich, ME;Conti, L;Cattaneo, E
ST 14 A细胞系先前通过逆转录病毒转导温度敏感的SV 40大T抗原从胚胎第14天大鼠纹状体原基衍生。我们发现，细胞分裂和巢蛋白的表达持续在33摄氏度，允许的温度，而细胞分裂停止，巢蛋白的表达减少，和MAP 2的表达增加，在39摄氏度的非允许温度。在这项研究中，我们进一步表征了细胞，发现它们表达其他一般和亚型特异性神经元特征，ST 14 A细胞表达烯醇化酶和β III-微管蛋白，此外，它们表达纹状体标志物DARPP-32，其在细胞分化时通过在无血清培养基中生长而上调。用多巴胺、DB-多巴胺受体激动剂喹吡罗或D1-多巴胺受体激动剂SKF 82958刺激导致CREB磷酸化。用刺激MAPK和腺苷酸环化酶途径的试剂的混合物处理细胞从根本上改变了ST 14 A细胞的形态。这些细胞发展出许多神经突样的突起，这些突起被β III-微管蛋白染色。此外，在这些条件下，细胞内注射矩形去极化电流刺激elpherins过冲动作电位与相对较快的去极化率时，从一个强烈的超极化膜电位。总之，这些数据意味着ST 14 A细胞系显示出中等大小的多刺神经元亚型的一些特征，并提供了一种新的工具来阐明参与中等大小的多刺神经元分化和疾病的途径和分子。(C)北京大学出版社.
The ST14A cell line was previously derived from embryonic day 14 rat striatal primordia by retroviral transduction of the temperature-sensitive SV40 large T antigen. We showed that cell division and expression of nestin persists at 33 degreesC, the permissive temperature, whereas cell division ceases, nestin expression decreases, and MAP2 expression increases at the nonpermissive temperature of 39 degreesC. In this study, we further characterized the cells and found that they express other general and subtype-specific neuronal characteristics, ST14A cells express enolase and beta III-tubulin, Furthermore, they express the striatal marker DARPP-32, which is up-regulated upon differentiation of the cells by growth in serum-free medium. Stimulation with dopamine, the DB-dopamine receptor agonist quinpirole, or the D1-dopamine receptor agonist SKF82958 results in phosphorylation of CREB. Treatment of the cells with a mixture of reagents which stimulate the MAPK and adenylyl cyclase pathways radically changes the morphology of the ST14A cells. The cells develop numerous neurite-like appearing processes which stain with beta III-tubulin. Moreover, under these conditions, intracellular injection of rectangular depolarizing current stimuli elicits overshooting action potentials with a relatively fast depolarization rate when starting from a strongly hyperpolarized membrane potential. Taken together, these data imply that the ST14A cell line displays some of the characteristics of a medium-size spiny neuron subtype and provides a new tool to elucidate the pathways and molecules involved in medium-size spiny neuron differentiation and disease. (C) 2000 Academic Press.