Tractable Cre-lox system for stochastic alteration of genes in mice
Tractable Cre-lox system for stochastic alteration of genes in mice
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DOI：
10.1038/nmeth.1183
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发表时间：
2008-03-01
期刊：
影响因子：
48
通讯作者：
Liskay, R. Michael
中科院分区：
文献类型：
--
作者：
Miller, Ashleigh J.;Dudley, Sandra D.;Liskay, R. Michael
我们开发了一种细胞分裂激活的Cre-lox系统，用于小鼠loxp侧位点的随机重组。通过移码逆转激活的Cre由DNA错配修复状态调节，发生在正常组织包围的单个细胞中，模仿自发的致癌突变。该系统对于描述肿瘤形成的途径，以及确定特定基因改变对发育和衰老的影响尤其有用。
We developed a cell division-activated Cre-lox system for stochastic recombination of loxP-flanked loci in mice. Cre activation by frameshift reversion is modulated by DNA mismatch-repair status and occurs in individual cells surrounded by normal tissue, mimicking spontaneous cancer-causing mutations. This system should be particularly useful for delineating pathways of neoplasia, and determining the developmental and aging consequences of specific gene alterations.