Distribution of silver nanoparticles in pregnant mice and developing embryos
Distribution of silver nanoparticles in pregnant mice and developing embryos
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DOI：
10.3109/17435390.2011.626539
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发表时间：
2012-12-01
期刊：
影响因子：
5
通讯作者：
Goering, Peter L.
中科院分区：
文献类型：
--
作者：
Austin, Carlye A.;Umbreit, Thomas H.;Goering, Peter L.
本研究的目的是评估银纳米粒子（NP）在怀孕小鼠及其发育胚胎中的分布。在妊娠第 7、8 和 9 天，将银纳米颗粒（平均直径 50 nm）以 0、35 或 66 μg Ag/小鼠的剂量水平静脉注射到妊娠 CD-1 小鼠中。 GD10 对小鼠实施安乐死，收集组织样本并分析银含量。与注射柠檬酸盐缓冲液载体的对照动物相比，银 NP 处理小鼠的几乎所有组织中的银含量均显着增加 (p < 0.05)。与银NP治疗小鼠的其他器官相比，肝脏、脾脏、肺、尾部（注射部位）、内脏卵黄囊和子宫内膜中的银积累显着更高。此外，在内脏卵黄囊内胚层细胞的囊泡中发现了银纳米颗粒。总之，结果表明银纳米粒子分布到大多数母体器官、胚胎外组织和胚胎中，但在胚胎中没有显着积累。
The objective of this study was to evaluate the distribution of silver nanoparticles (NPs) in pregnant mice and their developing embryos. Silver NPs (average diameter 50 nm) were intravenously injected into pregnant CD-1 mice on gestation days (GDs) 7, 8, and 9 at dose levels of 0, 35, or 66 mu g Ag/mouse. Mice were euthanised on GD10, and tissue samples were collected and analysed for silver content. Compared with control animals injected with citrate buffer vehicle, silver content was significantly increased (p < 0.05) in nearly all tissues from silver NP-treated mice. Silver accumulation was significantly higher in liver, spleen, lung, tail (injection site), visceral yolk sac, and endometrium compared with other organs from silver NP-treated mice. Furthermore, silver NPs were identified in vesicles in endodermal cells of the visceral yolk sac. In summary, the results demonstrated that silver NPs distributed to most maternal organs, extra-embryonic tissues, and embryos, but did not accumulate significantly in embryos.