Rational control of enantioselectivity of lipase by site-directed mutagenesis based on the mechanism
Rational control of enantioselectivity of lipase by site-directed mutagenesis based on the mechanism
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DOI：
10.1039/b508244g
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发表时间：
2005-01-01
影响因子：
4.9
通讯作者：
Sakai, T
中科院分区：
文献类型：
--
作者：
Ema, T;Fujii, T;Sakai, T
在此基础上，通过引入单一突变，洋葱伯克霍尔德菌脂肪酶对仲醇的对映选择性既可以增加，也可以合理地降低.
The enantioselectivity of a Burkholderia cepacia lipase toward secondary alcohols could be both increased and decreased rationally by introducing only a single mutation on the basis of the mechanism proposed previously.