Gluconeogenesis in rat hepatocytes from monomethyl succinate and other esters.
Gluconeogenesis in rat hepatocytes from monomethyl succinate and other esters.
复制标题
大鼠肝细胞中琥珀酸单甲酯和其他酯的糖异生。
DOI：
10.1016/0003-9861(84)90441-7
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发表时间：
1984
影响因子：
3.9
通讯作者：
Rognstad,R
中科院分区：
文献类型：
--
作者：
Rognstad,R
虽然琥珀酸在大鼠肝细胞中形成的葡萄糖很少，但琥珀酸单甲酯的葡萄糖生成速率接近l-乳酸。琥珀酸二甲酯在5 mm处与琥珀酸单甲酯一样好，但在20 mm处则不然。富马酸单乙酯和4-甲基苹果酸酯仅是一般的生糖底物，但1-甲基苹果酸酯在高浓度下是另一种良好的底物。这些酯显然直接通过细胞膜吸收，或被单羧酸转运蛋白吸收，然后被一些酯酶在细胞内水解。这种方法可以允许使用更广泛的底物和抑制剂的肝细胞代谢的研究。
Although little glucose is formed from succinate in rat hepatocytes, the rate of gluconeogenesis from monomethyl succinate approaches that froml-lactate. Dimethyl succinate is as good as monomethyl succinate at 5 mm, but not at 20 mm. Monoethyl fumarate and 4-methyl malate are only fair glucogenic substrates, but 1-methyl malate is another good substrate at high concentrations. The esters are apparently taken up either directly through the cell membrane, or by monocarboxylate transporters, and then hydrolyzed intracellularly by some esterase(s). This approach may permit the use of a wider range of substrates and inhibitors for the study of liver cell metabolism.