DOPAMINE-RECEPTOR MESSENGER-RNA EXPRESSION PATTERNS BY OPIOID PEPTIDE CELLS IN THE NUCLEUS-ACCUMBENS OF THE RAT - A DOUBLE IN-SITU HYBRIDIZATION STUDY
DOPAMINE-RECEPTOR MESSENGER-RNA EXPRESSION PATTERNS BY OPIOID PEPTIDE CELLS IN THE NUCLEUS-ACCUMBENS OF THE RAT - A DOUBLE IN-SITU HYBRIDIZATION STUDY
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DOI：
10.1002/cne.903610106
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发表时间：
1995-10-09
影响因子：
2.5
通讯作者：
WATSON, SJ
中科院分区：
文献类型：
--
作者：
CURRAN, EJ;WATSON, SJ
在大鼠伏隔核中分析了前脑啡肽和前啡肽mrna相互之间以及与D1、D2和D3多巴胺受体mrna的共定位。在伏隔核的吻侧极、核和壳细分中发现了不同的组合。Proenkephalin和prodynorphin mrna主要定位于核心的独立细胞中。核心区所有可检测到的前啡肽细胞均表达DI mRNA，而不表达D2 mRNA。相反，该区域约95%的前脑啡肽阳性细胞表达D2 mRNA，而不表达D1 mRNA。这种模式与在尾状壳核中观察到的相同。在吻极和壳中，嵌入在这种“典型”共定位模式的背景中，发现了表达独特结构的细胞群。在这些细胞簇中，前脑啡肽阳性细胞表达前啡肽和D1 mRNA，但不表达D2 mRNA。D3和prodynorphin mrna共定位于“边缘”纹状体区域，包括腹内侧尾状壳核、吻侧极和内侧壳。相比之下，在任何前脑啡肽阳性细胞中均未检测到D3 mRNA。结合prodynorphin/D1数据，这表明prodynorphin细胞的一个子集同时表达D1和D3 mrna。结果表明：1)共同表达前脑啡肽、前肌啡肽和D1 mrna的细胞簇与伏隔核中先前定义的细胞结构细胞簇广泛重叠；2)伏隔核腹内侧尾状壳核和伏隔核中的前肌啡肽细胞亚群同时含有D1和D3 mrna。(C) 1995 Wiley-Liss, Inc。
Colocalization of proenkephalin and prodynorphin mRNAs with each other as well as with D1, D2, and D3 dopamine receptor mRNAs was analyzed in the nucleus accumbens of the rat. Distinct combinations were detected in the rostral pole, core, and shell subdivisions of the nucleus accumbens. Proenkephalin and prodynorphin mRNAs were principally localized in separate cells in the core. All detectable prodynorphin cells in the core expressed DI mRNA but not D2 mRNA. Conversely, approximately 95% of the proenkephalin-positive cells in this region expressed D2 mRNA but not D1 mRNA. This pattern was identical to that observed in the caudate putamen. In the rostral pole and the shell, embedded in a background of this ''typical'' colocalization pattern, clusters of cells expressing a distinct configuration were found. In these clusters, proenkephalin-positive cells expressed both prodynorphin and D1 mRNAs, but they did not express D2 mRNA.D3 and prodynorphin mRNAs were colocalized in ''limbic'' striatal areas, including the ventromedial caudate putamen, the rostral pole, and the medial shell. In contrast, D3 mRNA was not detected in any proenkephalin-positive cells. Together with the prodynorphin/D1 data, this suggests that a subset of prodynorphin cells expresses both D1 and D3 mRNAs.It is concluded that 1) clusters of cells that coexpress proenkephalin, prodynorphin, and D1 mRNAs overlap extensively with previously defined cytoarchitectural cell clusters in the nucleus accumbens and 2) a subset of the prodynorphin cells in the ventromedial caudate putamen and the nucleus accumbens contains both D1 and D3 mRNAs. (C) 1995 Wiley-Liss, Inc.