A novel TaMYB4 transcription factor involved in the defence response against Puccinia striiformis f. sp tritici and abiotic stresses
A novel TaMYB4 transcription factor involved in the defence response against Puccinia striiformis f. sp tritici and abiotic stresses
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一种新型 TaMYB4 转录因子，参与针对条锈菌 f 的防御反应。
DOI：
10.1007/s11103-013-0156-7
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发表时间：
2014-03-01
影响因子：
5.1
通讯作者：
Kang, Zhensheng
中科院分区：
文献类型：
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作者：
Al-Attala, M. Nashaat;Wang, Xiaojie;Kang, Zhensheng
MYB转录因子是一个大的蛋白质家族，参与调控次级代谢和细胞形状，增强抗病能力和对不同胁迫的反应。本研究研究了TaMYB4在小麦抗生物和非生物胁迫中的作用。TaMYB4克隆自小麦cv。苏皖11株叶片侵染条纹锈病。tritici (Pst)];TaMYB4蛋白的长度为243个氨基酸。此外，TaMYB4与来自Brachypodium distachyon的BdMYB4具有高度的相似性，也被鉴定为R2R3-MYB家族的成员。瞬时表达分析表明，推导出的TaMYB4蛋白定位于洋葱表皮细胞的细胞核中。此外，酵母单杂交实验显示TaMYB4具有转录活性，并且c端是激活转录所必需的。直接观察到TaMYB4的转录本水平，发现在接种不相容的Pst后的早期和48 h显著上调。在小麦根、茎和叶片中均检测到TaMYB4转录本。此外，水杨酸、乙烯、脱落酸和茉莉酸甲酯均可诱导TaMYB4的转录。冷敷和创面处理的结果相同。此外，利用病毒诱导的基因沉默敲低TaMYB4的表达，增强了小麦品种苏圆11对Pst不亲和性小种的敏感性。这些结果表明，TaMYB4在小麦对生物胁迫的响应中起着重要作用。
MYB transcription factors are a large family of proteins involved in the regulation of secondary metabolism and cell shape, the enhancement of disease resistance and the response to different stresses. In this study, the role of TaMYB4 in wheat against biotic and abiotic stresses was investigated. TaMYB4 was cloned from wheat cv. Suwan11 [the leaves were infected with Puccinia striiformis f.sp. tritici (Pst)]; the TaMYB4 protein is 243 amino acids in length. In addition, TaMYB4 exhibited high similarity with BdMYB4 from Brachypodium distachyon, which was also identified as a member of the R2R3-MYB family of genes. Furthermore, transient expression analysis showed that the deduced TaMYB4 protein was localised in the nucleus of onion epidermal cells. Additionally, a yeast one-hybrid assay revealed that TaMYB4 exhibits transcriptional activity and the C-terminus is necessary for the activation of transcription. The transcript levels of TaMYB4 were observed directly and were found to be significantly upregulated in the early stage and 48 h after inoculation with the incompatible Pst. The transcripts of TaMYB4 were detected in the wheat roots, culms and leaves. Moreover, the transcription of TaMYB4 was induced by salicylic acid, ethylene, abscisic acid and methyl jasmonate hormones. The same results were obtained with cold and wound treatments. Furthermore, the knockdown of TaMYB4 expression using virus-induced gene silencing enhanced the susceptibility of wheat cultivar Suwon11 to the incompatible race of Pst. These results demonstrate that TaMYB4 plays a role in the wheat response to biotic stress.