Monoclonal infection involving Mycobacterium avium presenting with three distinct colony morphotypes.
Monoclonal infection involving Mycobacterium avium presenting with three distinct colony morphotypes.
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涉及鸟分枝杆菌的单克隆感染呈现出三种不同的菌落形态类型。
DOI：
10.1128/jcm.34.10.2475-2478.1996
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发表时间：
1996
影响因子：
9.4
通讯作者：
Barrow,WW
中科院分区：
文献类型：
--
作者：
Wright,EL;Zywno-vanGinkel,S;Rastogi,N;Barrow,WW
最近的报告表明，多克隆感染可能在鸟分枝杆菌感染的多重耐药性中发挥重要作用。我们在此报告了单个鸟分枝杆菌菌株的分离情况，该菌株在 Lowenstein-Jensen 斜面上初次分离时似乎具有光滑的菌落形态。然而，在 Middlebrook 7H10 上的原代继代培养显示出三种不同的形态类型，分别代表光滑不透明 (SmO)、光滑透明 (SmT) 和粗糙 (Rg) 菌落形态。通过标准生化程序、Genprobe 分析和分枝菌酸模式，所有三种形态型均被鉴定为鸟分枝杆菌。随后使用 SalI 和 PvuII 消化的基因组 DNA 进行的限制性片段长度多态性分析显示与 IS1245 探针的杂交模式相同。对三种形态类型的脂质进行薄层色谱分析表明，只有 SmT 形态类型具有与先前描述的血清型特异性糖肽脂质抗原相似但又不同的脂质成分。通过高效液相色谱和薄层色谱对来自SmT形态型的内部放射性标记的脱酰化脂质进行进一步分析，发现这些组分中的一些可以用[14C]苯丙氨酸和[14C]甘露糖进行内部放射性标记。这些结果表明这些成分在结构上与先前描述的糖肽脂抗原相似。这显然是首次报道涉及鸟分枝杆菌单一菌株的单克隆感染，该菌株具有所有三种菌落形态类型：SmO、SmT 和 Rg。
Recent reports indicate that polyclonal infections may play an important role in multiple drug resistance in Mycobacterium avium infections. We report here on the isolation of a single M. avium strain that appeared to have smooth colony morphology upon initial isolation on a Lowenstein-Jensen slant. Primary subculture onto Middlebrook 7H10, however, revealed three distinct morphotypes representing smooth opaque (SmO), smooth transparent (SmT), and rough (Rg) colony morphologies. All three morphotypes were identified as M. avium by standard biochemical procedures, Genprobe analysis, and mycolic acid patterns. Subsequent restriction fragment length polymorphism analysis, using SalI- and PvuII-digested genomic DNA, revealed identical patterns for hybridization with the IS1245 probe. Thin-layer chromatographic analysis of lipids from the three morphotypes revealed that only the SmT morphotype possessed what appeared to be lipid components similar to, but unlike, previously described serovar-specific glycopeptidolipid antigens. Further analysis of internally radiolabeled deacylated lipids from the SmT morphotype, by high-performance liquid chromatography and thin-layer chromatography, disclosed that some of these components can be internally radiolabeled with [14C] phenylalanine and [14C]mannose. These results suggest that these components are structurally similar to previously described glycopeptidolipid antigens. This is apparently the first report of a monoclonal infection involving a single strain of M. avium presenting with all three colony morphotypes, SmO, SmT, and Rg.