Opioid-mediated pain sensitivity in mice bred for high voluntary wheel running.
Opioid-mediated pain sensitivity in mice bred for high voluntary wheel running.
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阿片类药物介导的高自愿轮跑小鼠的疼痛敏感性。
DOI：
10.1016/j.physbeh.2004.09.003
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
GarlandJr,Theodore
中科院分区：
文献类型：
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作者：
Li,Guo;Rhodes,JustinS;Girard,Isabelle;Gammie,StephenC;GarlandJr,Theodore
我们测试了一个假设，即热甩尾潜伏期，啮齿动物疼痛敏感性的一个常见指标，将在小鼠品系中改变，这些小鼠被选择性地培育为高自愿的车轮运行行为。具体来说，我们预测，所选择的（High-Runner）品系相对于其对照（C;随机繁殖）品系将显示出降低的疼痛敏感性，并且对阻断阿片受体的药物的反应不同。我们首先比较了High-Runner和C雌性小鼠在白天（无轮访问）和晚上（有轮访问）的甩尾潜伏期。其次，我们比较了阿片拮抗剂纳洛酮（10 mg/kg，i. p.）在白天的甩尾潜伏期（没有车轮访问）。第三，我们比较了纳洛酮（5和10 mg/kg，i. p.）和纳洛酮，一种更持久的阿片类拮抗剂（0.1、1、5、10、50和100 mg/kg，i. p.），在自愿的车轮上奔跑。甩尾潜伏期在夜间较长（当小鼠在轮子上活动时），但来自High-Runner和C系的小鼠在白天或夜间没有差异。给药纳洛酮（10 mg/kg，i. p.）白天测量的甩尾潜伏期减少，在High-Runner和C小鼠中同样如此。纳洛酮（5和10 mg/kg，i. p.）和高剂量纳洛酮（50和100 mg/kg，i. p.）减少高跑者和C小鼠的轮跑。需要进一步的研究来确定其他类型的疼痛敏感性是否也未能与自愿轮运行的增加相关。
We tested the hypothesis that thermal tail-flick latency, a common measure of pain sensitivity in rodents, would be altered in lines of mice that had been selectively bred for high voluntary wheel-running behavior. Specifically, we predicted that the selected (High-Runner) lines would show decreased pain sensitivity relative to their control (C; randombred) lines, and would respond differently to drugs that block opioid receptors. We first compared tail-flick latency between High-Runner and C female mice during the day (no wheel access) and at night (with wheel access). Second, we compared effects of the opioid antagonist naloxone (10 mg/kg, i.p.) on tail-flick latency during the day (no wheel access). Third, we compared effects of naloxone (5 and 10 mg/kg, i.p.) and naltrexone, a longer-lasting opioid antagonist (0.1, 1, 5, 10, 50, and 100 mg/kg, i.p.), on voluntary wheel running. Tail-flick latencies were longer at night (when mice were active on wheels), but mice from High-Runner and C lines did not differ during the day or night. Administration of naloxone (10 mg/kg, i.p.) decreased tail-flick latency measured during the day, equally in High-Runner and C mice. Naloxone (5 and 10 mg/kg, i.p.) and high doses of naltrexone (50 and 100 mg/kg, i.p.) decreased wheel running equally in High-Runner and C mice. Further studies will be required to determine whether other types of pain sensitivity have also failed to evolve in association with increased voluntary wheel running.