Major groove accessibility of RNA.
Major groove accessibility of RNA.
复制标题
DOI：
10.1126/science.7690496
复制
发表时间：
1993-09
期刊：
影响因子：
56.9
通讯作者：
Kevin M. Weeks;Donald M. Crothers
中科院分区：
文献类型：
--
作者：
Kevin M. Weeks;Donald M. Crothers
化学酰化实验表明，RNA大沟，通常被认为是太深，太窄，以允许识别相互作用，是在双链体末端访问。反应性在5'方向上比在3'方向上进一步延伸到螺旋中。螺旋之间的不对称和大环使它们解偶联，与相同大小的小环或对称环相比，这产生了在末端碱基对处增强的反应性和较弱的稳定化焓。解偶联的螺旋具有有效的螺旋末端，其具有可接近的主要凹槽;这样的基序对于蛋白质识别、三级折叠和催化是有吸引力的贡献者。
Chemical acylation experiments showed that the RNA major groove, often assumed to be too deep and narrow to permit recognition interactions, is accessible at duplex termini. Reactivity extended further into the helix in the 5' than in the 3' direction. Asymmetric and large loops between helices uncoupled them, which yielded both enhanced reactivity at terminal base pairs and weaker stabilization enthalpy compared to that in small loops or symmetric loops of the same size. Uncoupled helices have effective helix ends with accessible major grooves; such motifs are attractive contributors to protein recognition, tertiary folding, and catalysis.