Coexpression of carcinoembryonic antigen and E-cadherin in colorectal adenocarcinoma with liver metastasis
Coexpression of carcinoembryonic antigen and E-cadherin in colorectal adenocarcinoma with liver metastasis
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DOI：
10.1016/j.gassur.2003.07.001
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发表时间：
2003-11-01
影响因子：
3.2
通讯作者：
Kim, JS
中科院分区：
文献类型：
--
作者：
Kim, JC;Roh, SA;Kim, JS
癌胚抗原（CEA）被认为是结直肠癌的转移激活因子，而E-钙粘蛋白的表达在多种癌中下调。CEA和E-cadherin的表达同时进行了评估，在不同部位的结直肠癌肝转移的肿瘤进展。连续26例结直肠癌肝转移患者接受了原发肿瘤和肝转移的根治性手术。免疫组化染色采用标记链霉亲和素-生物素法检测CEA和E-cadherin的表达。采用一步法逆转录-聚合酶链反应（RT-PCR）原位RT PCR检测其mRNA表达。原发灶和肝转移灶癌中心CEA和E-cadherin表达评分均高于癌边缘（P < 0.001 ~ 0.006）。CEA表达评分与相应肿瘤部位E-cadherin表达评分密切相关（P < 0.001 ~ 0.017）。CEA和E-cadherin mRNA在癌旁肝细胞中表达最高，原发灶次之，转移灶最低（P < 0.001 ~ 0.002）。癌胚抗原（CEA）mRNA和上皮钙粘素（E-cadherin）mRNA在原发灶和癌旁肝细胞中的表达也呈显著正相关（P < 0.001），而癌旁肝细胞中的表达则呈显著负相关（P = 0.018）。肝转移边缘CEA表达评分较低的患者似乎比CEA表达评分较高的患者有更长的无病生存期。CEA和E-cadherin的表达与mRNA水平密切相关。此外，考虑到它们的生物学特性，这些相关性可能与结直肠癌的肿瘤进展有关。(C)2003年消化道外科学会。
Carcinoembryonic antigen (CEA) has been suggested as a metastatic activator in colorectal carcinoma, whereas the E-cadherin expression is downregulated in a variety of carcinomas. CEA and E-cadherin expressions were simultaneously assessed with regard to tumor progression in the various sites of colorectal carcinomas with liver metastasis. Twenty-six consecutive patients who had colorectal carcinoma with liver metastasis underwent curative surgery for primary tumor and liver metastasis. CEA and E-cadherin expression were identified on immunohistochemical staining using the labeled streptavidin-biotin method. Their mRNA expression was also detected by RT in situ PCR using one-step reverse transcription-polymerase chain reaction (RT-PCR). CEA and E-cadherin expression scores in the tumor center were greater than those in the tumor margin in both primary tumor and liver metastasis (P < 0.001 to 0.006). CEA expression scores were closely associated with E-cadherin expression scores on the corresponding tumor site (P < 0.001 to 0.017). CEA and E-cadherin mRNA expression was greatest in the hepatocytes adjacent to liver metastasis, next greatest in the primary tumor, and least in the liver metastasis (P < 0.001 to 0.002). CEA mRNA expression was also closely correlated with E-cadherin mRNA expression in the primary tumor (P < 0.001) and in the adjacent hepatocytes of the liver metastasis (P = 0.018). Patients with a lesser CEA expression score in the liver metastasis margin appeared to have a longer disease-free survival period than did those with a greater CEA expression score. Expression of CEA and E-cadherin was closely correlated with the mRNA levels. Furthermore, these correlations may be implicated in the tumor progression of colorectal carcinoma considering their biological properties. (C) 2003 The Society for Surgery of the Alimentary Tract.