High-resolution mapping of H4K16 and H3K23 acetylation reveals conserved and unique distribution patterns in Arabidopsis and rice
High-resolution mapping of H4K16 and H3K23 acetylation reveals conserved and unique distribution patterns in Arabidopsis and rice
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DOI：
10.1080/15592294.2015.1104446
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发表时间：
2015-11-02
期刊：
影响因子：
3.7
通讯作者：
Zhong, Xuehua
中科院分区：
文献类型：
--
作者：
Lu, Li;Chen, Xiangsong;Zhong, Xuehua
组蛋白乙酰化和去乙酰化是真核生物中重要的表观遗传基因调控机制。组蛋白4赖氨酸16（H4K16ac）的乙酰化涉及许多细胞过程。然而，它的生物学功能和与转录的关系在植物中很大程度上是未知的。我们在拟南芥和水稻中首次构建了H4K16ac的全基因组高分辨率图谱。我们发现H4K16ac优先富集在转录起始位点周围，并与基因表达水平呈正相关。H4K16ac和H3K23ac的共存与高基因表达水平相关，表明H4K16ac和H3K23ac组蛋白3赖氨酸23乙酰化对基因表达的潜在组合效应。我们的数据进一步揭示，虽然富含H4K16ac和H3K23ac的基因普遍表达，但仅富含H4K16ac或H3K23ac的基因显示出与特定发育阶段相关的显著不同的表达模式。出乎意料的是，与拟南芥不同，在水稻中低表达的基因中存在显著水平的H4K16ac和H3K23ac。此外，我们发现H4K16ac富集基因与拟南芥和水稻中不同的生物学过程相关，这表明H4K16ac在植物中可能具有种特异性作用。总之，我们的全基因组分析揭示了H4K16ac和H3K23ac在拟南芥和水稻中保守和独特的分布模式，并为进一步了解它们在植物中的功能提供了基础。
Histone acetylation and deacetylation are key epigenetic gene regulatory mechanisms that play critical roles in eukaryotes. Acetylation of histone 4 lysine 16 (H4K16ac) is implicated in many cellular processes. However, its biological function and relationship with transcription are largely unexplored in plants. We generated first genome-wide high-resolution maps of H4K16ac in Arabidopsis thaliana and Oryza sativa. We showed that H4K16ac is preferentially enriched around the transcription start sites and positively correlates with gene expression levels. Co-existence of H4K16ac and H3K23ac is correlated with high gene expression levels, suggesting a potentially combinatorial effect of H4K16ac and H3K23ac histone 3 lysine 23 acetylation on gene expression. Our data further revealed that while genes enriched with both H4K16ac and H3K23ac are ubiquitously expressed, genes enriched with only H4K16ac or H3K23ac showed significantly distinct expression patterns in association with particular developmental stages. Unexpectedly, and unlike in Arabidopsis, there are significant levels of both H4K16ac and H3K23ac in the lowly expressed genes in rice. Furthermore, we found that H4K16ac-enriched genes are associated with different biological processes in Arabidopsis and rice, suggesting a potentially species-specific role of H4K16ac in plants. Together, our genome-wide profiling reveals the conserved and unique distribution patterns of H4K16ac and H3K23ac in Arabidopsis and rice and provides a foundation for further understanding their function in plants.