Evaluation of a stereotactic frame for repositioning of the rat brain in serial positron emission tomography imaging studies
Evaluation of a stereotactic frame for repositioning of the rat brain in serial positron emission tomography imaging studies
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DOI：
10.1016/s0165-0270(01)00352-1
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发表时间：
2001-05-30
影响因子：
3
通讯作者：
Cherry, SR
中科院分区：
文献类型：
--
作者：
Rubins, DJ;Meadors, AK;Cherry, SR
在用正电子发射断层扫描(PET)对大鼠脑进行系列成像研究时，头部的可重复定位有助于图像的空间对齐和定量分析。为了实现这一目标，我们构建了一个塑料头架，并在高分辨率小动物PET扫描仪microPET上测试了定位的重复性。对两组锥度为18度(锐角)或45度(钝度)的耳杆进行了评估，以确定它们在将动物固定到框架上的相对精度。对于动物的顺序定位，分别用尖耳杆和钝耳杆测量距平均位置0.51 mm(SD 0.41 mm)和0.91 mm(SD 0.48 mm)的平均距离。这些结果表明，使用该框架可以重复定位大鼠的大脑，位置的变化小于现代动物PET扫描仪的空间分辨率。在一次扫描上定义的感兴趣脑区，并在随后的帧重新定位动物扫描中复制，结果显示使用尖尖耳杆的平均变异系数为5.4%(SD 2.7%)，使用钝的耳杆的平均变异系数为6.8%(SD 2.5%)。这种方法有可能改善对系列PET研究的定量评估。(C)2001 Elsevier Science B.V.保留所有权利。
For serial imaging studies of the rat brain with positron emission tomography (PET), reproducible positioning of the head can facilitate spatial alignment of images and quantitative analysis. To achieve this aim, we constructed a plastic head frame and tested the positioning reproducibility on a high-resolution small-animal PET scanner, microPET. Two sets of ear bars, with tapers of either 18 degrees (sharp) or 45 degrees (blunt), were evaluated for their relative precision in securing the animal to the frame. For sequential positioning of an animal, average distances from the mean position of 0.51 mm (SD 0.41 mm) and 0.91 mm (SD 0.48 mm) were measured with the sharp and blunt ear bars, respectively. These results show that a rat brain can be reproducibly positioned using the frame, with a variation of position less than the spatial resolution of modern animal PET scanners. Brain regions of interest defined on one scan and copied across subsequent scans of a frame-repositioned animal resulted in an average coefficient of variation of 5.4% (SD 2.7%) using the sharp ear bars and 6.8% (SD 2.5%) using the blunt ear bars. This methodology has the potential to improve quantitative assessment for serial PET studies. (C) 2001 Elsevier Science B.V. All rights reserved.