A simple analytical method involving the use of a monolithic silica disk-packed spin column and HPLC-ECD for determination of L-DOPA in plasma of patients with Parkinson's disease
A simple analytical method involving the use of a monolithic silica disk-packed spin column and HPLC-ECD for determination of L-DOPA in plasma of patients with Parkinson's disease
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DOI：
10.1039/c3ay40934a
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发表时间：
2013-09
影响因子：
3.1
通讯作者：
M. Tsunoda;M. Hirayama;K. Ohno;T. Tsuda
中科院分区：
文献类型：
--
作者：
M. Tsunoda;M. Hirayama;K. Ohno;T. Tsuda
L-DOPA （l -3,4-二羟基苯丙氨酸）通常用于治疗帕金森病。监测人血浆中左旋多巴的浓度将使剂量优化成为可能，但由于目前的定量方法繁琐且耗时，因此很少进行。本研究建立了一种简便的测定帕金森病患者血浆中左旋多巴的方法。采用含有苯硼酸酯的硅胶圆盘填充自旋柱，与左旋多巴的顺羟基形成稳定的阴离子配合物，从血浆中提取左旋多巴，萃取回收率超过90%。提取的L-DOPA在反相柱上分离，用掺杂硼的金刚石电极进行电化学检测。该方法的检出限为10 fmol，成功地应用于健康志愿者和帕金森病患者血浆中左旋多巴的测定。
L-DOPA (L-3,4-dihydroxyphenylalanine) is commonly used in the treatment of Parkinson's disease. Monitoring the concentration of L-DOPA in human plasma will enable dose optimization, but is rarely performed because current quantification methods are tedious and time-consuming. In this study, a simple method for the determination of L-DOPA in the plasma of patients with Parkinson's disease was developed. A monolithic silica disk-packed spin column with a phenylboronate moiety, which forms stable anionic complexes with the cis-hydroxyl groups of L-DOPA, was used to extract L-DOPA from plasma with extraction recoveries exceeding 90%. The extracted L-DOPA was then separated on a reversed-phase column and detected electrochemically with a boron-doped diamond electrode. The method, which has a limit of detection of 10 fmol, was then successfully applied for the determination of L-DOPA in the plasma of healthy volunteers and patients with Parkinson's disease.