Application of Hepatitis E Virus-Related Markers on Samples from a Developing Country
Application of Hepatitis E Virus-Related Markers on Samples from a Developing Country
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戊型肝炎病毒相关标记在发展中国家样本中的应用
DOI：
10.7754/clin.lab.2018.180708
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发表时间：
2019
影响因子：
0.7
通讯作者：
Ningshao Xia
中科院分区：
文献类型：
--
作者：
Si-Ling Wang;Birendra Prasad Gupta;Wen-Fang Ji;Zi-Min Tang;Gui-Ping Wen;Anurag Adhikari;Dong Ying;Xu Zhang;Chang Liu;Zizheng Zheng;Ningshao Xia
尼泊尔是戊型肝炎病毒（HEV）流行病的流行地区。2014年4月至5月在尼泊尔Biratnagar收集了170名出现肝炎症状的患者的血清样本，然后将其运送到中国厦门进行进一步评估。所有标本均检测HEVRNA、HEV抗原、抗-HEVIgM、抗-HEVIgG、抗-HBcIgM、抗-HCVIgG、抗-HAVIgM。戊型肝炎是潜在活动性肝炎的主要病因（59.2%，16/27），其次是乙型肝炎（25.9%，7/27，抗-HBc IgM阳性），甲型肝炎（18.5%，5/27，抗-HAV IgM阳性）和丙型肝炎（3.7%，1/27，抗-HCV抗体）。16例确诊的HE均为HEV抗原阳性，5例HEVRNA阳性，15例抗-HEVIgM阳性。RNA阳性率低可能与这些样本的采集和/或运输有关。本研究表明，戊型肝炎病毒是发展中国家和地区急性肝炎的主要原因。免疫诊断试剂盒的应用，特别是HEV抗原检测，在这些国家和地区显示了巨大的潜力。
Nepal is an endemic area for hepatitis E virus (HEV) epidemics. The research on viral hepatitis in Nepal is limited.Serum samples from 170 patients presenting with symptoms of hepatitis were collected from April to May 2014 in Biratnagar, Nepal, and then transported to Xiamen, China, for further evaluation. All samples were tested for HEV RNA, HEV antigen, anti-HEV IgM, anti-HEV IgG and anti-HBc IgM, anti-HCV IgG, and anti-HAV IgM.Sixteen patients were identified as acute hepatitis E with the presence of ≥ 2 HEV acute phase markers (antigen, RNA, and anti-HEV IgM). HEV infection was the major cause of potential active viral hepatitis (59.2%, 16 of 27), followed by HBV (25.9%, 7 of 27, anti-HBc IgM positive), HAV (18.5%, 5 of 27, anti-HAV IgM positive), and HCV (3.7%, 1 of 27, anti-HCV antibodies). All 16 confirmed HE cases were positive for HEV antigen, while 5 cases were HEV RNA positive, as well as 15 anti-HEV IgM positive. The low positive rate of RNA might be related to the collection and/or the transportation of these samples.This study showed that HEV is a major cause of acute hepatitis in developing countries and regions. Application of immunoassay diagnostic kits, especially the HEV antigen tests, showed great potential for HE detection in these countries and regions.