Native mRNA editing complexes from Trypanosoma brucei mitochondria.
Native mRNA editing complexes from Trypanosoma brucei mitochondria.
复制标题
来自布氏锥虫线粒体的天然 mRNA 编辑复合物。
DOI：
10.1002/j.1460-2075.1992.tb05543.x
复制
发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Hajduk,SL
中科院分区：
文献类型：
--
作者：
Pollard,VW;Harris,ME;Hajduk,SL
本研究的目的是鉴定参与着丝质体线粒体mRNA编辑的多组分复合物。以10-30%甘油梯度对布鲁氏锥虫线粒体提取物进行分离，并检测可能参与编辑的RNA和活性，包括预编辑mRNA、引导RNA （gRNA）、核酸内切酶、末端脲基转移酶（TUTase）、RNA连接酶和gRNA - mRNA嵌合体形成活性。这些实验表明存在两种不同的编辑复合体。复合体I （19S）由gRNA、TUTase、RNA连接酶和嵌合体形成活性组成。复合物II （35-40S）由gRNA、预编辑mRNA、RNA连接酶和嵌合体形成活性组成。这些研究提供了第一个证据，证明编辑发生在多组分复合体中。讨论了复合体I、复合体II和RNA连接酶在编辑中的可能作用。
The aim of this study was to identify multicomponent complexes involved in kinetoplastid mitochondrial mRNA editing. Mitochondrial extracts from Trypanosoma brucei were fractionated on 10–30% glycerol gradients and assayed for RNAs and activities potentially involved in editing, including pre‐edited mRNA, guide RNA (gRNA), endonuclease, terminal uridylyltransferase (TUTase), RNA ligase and gRNA‐mRNA chimera‐forming activities. These experiments suggest that two distinct editing complexes exist. Complex I (19S) consists of gRNA, TUTase, RNA ligase and chimera‐forming activity. Complex II (35–40S) is composed of gRNA, preedited mRNA, RNA ligase and chimera‐forming activity. These studies provide the first evidence that editing occurs in a multicomponent complex. The possible roles of complex I, complex II and RNA ligase in editing are discussed.