Liquid chromatography, electrochromatography and capillary electrophoresis applications of DNA and RNA aptamers.
Liquid chromatography, electrochromatography and capillary electrophoresis applications of DNA and RNA aptamers.
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DOI：
10.1016/j.chroma.2006.03.101
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发表时间：
2006-06
期刊：
影响因子：
--
通讯作者：
C. Ravelet;C. Grosset;E. Peyrin
中科院分区：
文献类型：
--
作者：
C. Ravelet;C. Grosset;E. Peyrin
通过指数富集配体系统进化（SELEX）过程的发展使得寡核苷酸序列（称为适体）的分离能够以高亲和力和特异性识别各类目标分子。目前，各种基于适配体的分析格式已被开发为检测和定量众多靶标的合适工具，与最流行的抗体技术相比具有许多潜在优势。在这篇综述中，讨论了适体在通过液相色谱、毛细管电泳和电色谱分离、纯化和定量各种分析物中的适用性。
The development of the systematic evolution of ligands by exponential enrichment (SELEX) process has allowed the isolation of oligonucleotide sequences (referred as aptamers) with the capacity to recognize various classes of target molecules with high affinity and specificity. At the present time, various analytical aptamer-based formats have been developed as suitable tools for the detection and quantification of numerous targets, possessing many potential advantages over the most popular antibody technology. In this review, the aptamer applicability to the separation, purification and quantification of various analytes by liquid chromatography, capillary electrophoresis and electrochromatography is addressed.