Clonal analysis using recombinant DNA probes from the X-chromosome.
Clonal analysis using recombinant DNA probes from the X-chromosome.
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DOI：
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发表时间：
1987-09
期刊：
影响因子：
11.2
通讯作者：
B. Vogelstein;E. Fearon;S. Hamilton;A. C. Preisinger;H. Willard;A. Michelson;A. Riggs;S. Orkin
中科院分区：
文献类型：
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作者：
B. Vogelstein;E. Fearon;S. Hamilton;A. C. Preisinger;H. Willard;A. Michelson;A. Riggs;S. Orkin
已经证明，x染色体基因的限制性片段长度多态性可以与甲基化模式结合使用，以确定人类肿瘤的克隆组成。在本报告中，我们展示了几种x染色体探针可以用于这种分析。特别是，从次黄嘌呤磷酸核糖基转移酶基因和磷酸甘油酸激酶基因衍生的探针可用于50%以上的美国女性的克隆分析。用这些探针观察到的x失活模式被发现准确地反映了92个测试肿瘤中95%以上的克隆性。
It has been demonstrated that restriction fragment length polymorphisms of X-chromosome genes can be used in conjunction with methylation patterns to determine the clonal composition of human tumors. In this report, we show that several X-chromosome probes can be used for such analyses. In particular, probes derived from the hypoxanthine phosphoribosyltransferase gene and the phosphoglycerate kinase gene could be used for clonal analysis in over 50% of American females. The X-inactivation patterns observed with these probes were found to accurately reflect clonality in more than 95% of 92 tumors tested.