Emergency diagnosis made easy: matrix removal and analyte enrichment from raw saliva using paper-arrow mass spectrometry
Emergency diagnosis made easy: matrix removal and analyte enrichment from raw saliva using paper-arrow mass spectrometry
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DOI：
10.1039/d3an00850a
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发表时间：
2023-09-04
期刊：
影响因子：
4.2
通讯作者：
Maher，Simon
中科院分区：
文献类型：
--
作者：
Zhou，Yufeng;Sham，Tung-Ting;Maher，Simon
扑热息痛过量是急性肝功能衰竭的主要原因，可能被证明是致命的。准确、快速地建立扑热息痛浓度至关重要。目前的检测方法是有创的、耗时的和/或昂贵的。迫切需要非侵入性、快速和低成本的技术。为了应对这一挑战，人们开发了一种新的方法，称为纸质谱(PA-MS)。这项技术将样本采集、提取、浓缩、分离和电离整合到一张纸条上，从样本到结果的整个分析过程可以在不到10分钟的时间内完成，只需要2μL的原始人类唾液。PA-MS在4种浓度下的检出限为185ngmL−1，平均回收率为10 7±7%，平均准确度为11±8%，精密度≤为5%，在0.2~2 0 0μg m L−1浓度范围内具有良好的线性关系(R2=0.9988)，超过了目前用于扑热息痛分析的同类方法。此外，从一组人类唾液样本中，使用这种方法发现个体间的变异性为10%。这项技术为快速、准确的紧急诊断提供了一种很有前途的工具。
Paracetamol overdose is a leading cause of acute liver failure that can prove fatal. Establishing paracetamol concentration accurately and quickly is critical. Current detection methods are invasive, time-consuming and/or expensive. Non-invasive, rapid and cost-effective techniques are urgently required. To address this challenge, a novel approach, called Paper-Arrow Mass Spectrometry (PA-MS) has been developed. This technique combines sample collection, extraction, enrichment, separation and ionisation onto a single paper strip, and the entire analysis process, from sample to result, can be carried out in less than 10 min requiring only 2 μL of raw human saliva. PA-MS achieved a LOQ of 185 ng mL−1, mean recovery of 107 ± 7%, mean accuracy of 11 ± 8% and precision ≤5% using four concentrations, and had excellent linearity (r2 = 0.9988) in the range of 0.2–200 μg mL−1 covering the treatment concentration range, surpassing the best-in-class methods currently available for paracetamol analysis. Furthermore, from a panel of human saliva samples, inter-individual variability was found to be <10% using this approach. This technique represents a promising tool for rapid and accurate emergency diagnosis.