Moment analysis for kinetics of gene silencing by RNA interference
Moment analysis for kinetics of gene silencing by RNA interference
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DOI：
10.1002/bit.20718
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发表时间：
2006-03-05
影响因子：
3.8
通讯作者：
Takakura, Y
中科院分区：
文献类型：
--
作者：
Takahashi, Y;Yamaoka, K;Takakura, Y
从动力学角度定量评价RNA干扰（RNAi）。提出了一个简单的动力学评价矩分析的基础上，假设抑制和恢复阶段的基因表达。我们将抑制作用曲线下面积（AUC（IE））定义为RNAi总强度的指数，将抑制作用的平均响应时间（MRTIE）定义为其持续时间的指数。所提出的动力学分析有助于以定量和时间依赖的方式理解RNAi效应，这将有利于设计用于实验和治疗目的的基于RNAi的基因沉默。(c)2005 Wiley Periodicals，Inc.
RNA interference (RNAi) was quantitatively evaluated from a kinetic viewpoint. A simple kinetic evaluation based on moment analysis was proposed, assuming suppression and recovery phases of gene expression. We defined the area under the curve of the inhibitory effect (AUC(IE)) as an index of the total intensity of RNAi and the mean response time of the inhibitory effect (MRTIE) as an index of its duration. The proposed kinetic analysis helps to understand the RNAi effect in a quantitative and time-dependent manner, which will be beneficial for designing RNAi-based gene silencing for both experimental and therapeutic purposes. (c) 2005 Wiley Periodicals, Inc.