INTENSITY FLUCTUATION SPECTROSCOPY OF LASER LIGHT SCATTERED BY SOLUTIONS OF SPHERICAL VIRUSES - R17, QBETA, BSV, PM2, AND T7 .2. DIFFUSION-COEFFICIENTS, MOLECULAR-WEIGHTS, SOLVATION, AND PARTICLE DIMENSIONS
INTENSITY FLUCTUATION SPECTROSCOPY OF LASER LIGHT SCATTERED BY SOLUTIONS OF SPHERICAL VIRUSES - R17, QBETA, BSV, PM2, AND T7 .2. DIFFUSION-COEFFICIENTS, MOLECULAR-WEIGHTS, SOLVATION, AND PARTICLE DIMENSIONS
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DOI：
10.1021/bi00702a021
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发表时间：
1974-01-01
期刊：
影响因子：
2.9
通讯作者：
FRANKLIN, RM
中科院分区：
文献类型：
--
作者：
CAMERINI.RD;PUSEY, PN;FRANKLIN, RM
拉斐尔·D Camerini-Obery，* Peter N.放大图片作者：J. Koppel，§ Dale W. Schaefer，If和RichardM.富兰克林！|用强度涨落光谱法测定了几种球形病毒的扩散系数D。细菌病原体R_（17）、Q/3、PM_2和T_7的γ_（20），w值分别为1.534±0.015、1.423±0.014、0.650±0.007和0.644±0.007，植物病毒番茄丛矮病毒（BSV）的γ_（20），w值为1.246±0.012。根据这些D值结合沉降系数的文献值和我们测定的表观比容值（R17、Q/3和PM 2）或从文献中获得的表观比容值（BSV和T7）计算分子量。R17、Q/3、BSV、PM2和T7的计算分子量分别为（百万道尔顿）：4.02±0.17、4.55±0.16、8.81±0.17、47.9±1.7和50.9±1.1。这些值与文献值和估计值吻合良好
Rafael D. Camerini-Otero,* Peter N. Pusey, J Dennis E. Koppel, § Dale W. Schaefer, If and RichardM. Franklin!| abstract: The diffusion coefficients (D) of several spherical viruses have been determined by the method of intensity fluctuation spectroscopy. The values for 7> 2o, w for bacterio-phages R17, Q/3, PM2, and T7 are (in units of 10~ 7 cm2 sec"" 1): 1.534±0.015, 1.423±0.014, 0.650±0.007, and 0.644±0.007, respectively; that for the plant virus, tomato bushy stunt virus (BSV), is 1.246±0.012. The molecular weights were calculated from these values of D combined with litera-ture values for the sedimentation coefficients (s) and values for the apparent specific volumes determined by us (R17, Q/3, and PM2) or obtained from the literature (BSV and T7). The calculated molecular weights for R17, Q/3, BSV, PM2, and T7 are (in millions of daltons): 4.02±0.17, 4.55±0.16, 8.81±0.17, 47.9±1.7, and 50.9±1.1, respectively. These values are in good agreement with literature values and those estimated