Widespread premature transcription termination of Arabidopsis thaliana NLR genes by the spen protein FPA
Widespread premature transcription termination of Arabidopsis thaliana NLR genes by the spen protein FPA
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DOI：
10.1101/2020.12.15.422694
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发表时间：
2020-12
期刊：
影响因子：
7.7
通讯作者：
Matthew T. Parker;Katarzyna Knop;Vasiliki Zacharaki;Anna V. Sherwood;Daniel Tomé;Xuhong Yu;Pascal Martin;J. Beynon;S. Michaels;G. Barton;G. Simpson
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作者：
Matthew T. Parker;Katarzyna Knop;Vasiliki Zacharaki;Anna V. Sherwood;Daniel Tomé;Xuhong Yu;Pascal Martin;J. Beynon;S. Michaels;G. Barton;G. Simpson
参与抗病性的基因是基因组中进化最快、最多样化的组成部分之一。在植物基因组中发现了大量与核苷酸结合的富含亮氨酸重复序列的受体（NLR）基因，这些基因是植物抗病所必需的。然而，NLR可以引发自身免疫，破坏有益的微生物群或降低健康。因此，了解如何控制NLR至关重要。在这里，我们表明，RNA结合蛋白FPA介导广泛的过早切割和多聚腺苷酸化的NLR转录本，从而控制其功能表达和影响免疫。使用长读段纳米孔直接RNA测序，我们解决了NLR转录处理和基因注释的复杂性。我们的研究结果揭示了一个共转录层的NLR控制与理解的调节和进化动力学的NLR在植物的免疫反应的影响。
Genes involved in disease resistance are some of the fastest evolving and most diverse components of genomes. Large numbers of nucleotide-binding, leucine-rich repeat receptor (NLR) genes are found in plant genomes and are required for disease resistance. However, NLRs can trigger autoimmunity, disrupt beneficial microbiota or reduce fitness. It is therefore crucial to understand how NLRs are controlled. Here we show that the RNA-binding protein FPA mediates widespread premature cleavage and polyadenylation of NLR transcripts, thereby controlling their functional expression and impacting immunity. Using long-read Nanopore direct RNA sequencing, we resolved the complexity of NLR transcript processing and gene annotation. Our results uncover a co-transcriptional layer of NLR control with implications for understanding the regulatory and evolutionary dynamics of NLRs in the immune responses of plants.