Genome-wide analysis of the odorant-binding protein gene family in Drosophila melanogaster
Genome-wide analysis of the odorant-binding protein gene family in Drosophila melanogaster
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DOI：
10.1101/gr.239402
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发表时间：
2002-09-01
期刊：
影响因子：
7
通讯作者：
Tanouye, MA
中科院分区：
文献类型：
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作者：
Hekmat-Scafe, DS;Scafe, CR;Tanouye, MA
嗅觉对许多昆虫的生存和繁殖行为至关重要，包括食物来源的定位，配偶的选择，群体同种的识别，以及产卵场所的确定。昆虫嗅觉系统的一个普遍存在但知之甚少的组成部分是一组气味结合蛋白（OBP），它们以高浓度存在于嗅觉受体神经元树突周围的水性淋巴液中。OBP被认为是将气味从环境中穿梭到潜在的气味受体，它们可能潜在地充当气味呈递者。在这里，我们表明，果蝇基因组携带51个潜在的OF基因，其气味受体基因的数量相当。我们发现，大多数（73%）的这些OBP样基因发生在集群多达9个基因，在已观察到的果蝇气味受体基因。两个假定的OBP基因簇各自携带气味受体基因。我们还报告了一个有趣的亚家族12个假定的OBP共享一个独特的C-末端结构与三个保守的半胱氨酸和一个保守的脯氨酸。这个亚科的成员以前没有被描述为任何昆虫。我们已经进行了系统发育分析的OBP相关蛋白质在果蝇以及其他昆虫，我们讨论了这个大家庭的基因的重复和分歧。
Olfaction is of considerable importance to many insects in behaviors critical for survival and reproduction, including location of food sources, selection of mates, recognition of colony con-specifics, and determination of oviposition sites. An ubiquitous, but poorly understood, component of the insect's olfactory system is a group of odorant-binding proteins (OBPs) that are present at high concentrations in the aqueous lymph surrounding the dendrites of olfactory receptor neurons. OBPs are believed to shuttle odorants from the environment to the underlying odorant receptors, for which they could potentially serve as odorant presenters. Here we show that the Drosophila genome carries 51 potential OF genes, a number comparable to that of its odorant-receptor genes. We find that the majority (73%) of these OBP-like genes occur in clusters of as many as nine genes, in contrast to what has been observed for the Drosophila odorant-receptor genes. Two of the presumptive OBP gene clusters each carries an odorant-receptor gene. We also report an intriguing subfamily of 12 putative OBPs that share a unique C-terminal structure with three conserved cysteines and a conserved praline. Members of this subfamily have not previously been described for any insect. We have performed phylogenetic analyses of the OBP-related proteins in Drosophila as well as other insects, and we discuss the duplication and divergence of the genes for this large family.