Neuropeptide Receptors NPR-1 and NPR-2 Regulate Caenorhabditis elegans Avoidance Response to the Plant Stress Hormone Methyl Salicylate
Neuropeptide Receptors NPR-1 and NPR-2 Regulate Caenorhabditis elegans Avoidance Response to the Plant Stress Hormone Methyl Salicylate
复制标题
神经肽受体 NPR-1 和 NPR-2 调节秀丽隐杆线虫对植物应激激素水杨酸甲酯的回避反应
DOI：
10.1534/genetics.114.172239
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发表时间：
2015-02-01
期刊：
影响因子：
3.3
通讯作者：
Ma, Long
中科院分区：
文献类型：
--
作者：
Luo, Jintao;Xu, Zhaofa;Ma, Long
水杨酸甲酯(MESA)是植物在病原体或食草动物攻击下释放的一种应激激素。梅萨已被证明可以吸引草食动物的捕食性昆虫，并击退害虫。动物对MESA的反应背后的分子和神经元尚不清楚。在这里，我们发现线虫对MESA表现出强烈的回避反应，这需要两个密切相关的神经肽受体NPR-1和NPR-2的活性。分子分析表明，NPR-1在RMG间/运动神经元的表达是MESA回避所必需的。还需要NPR-1配体FLP-18。利用一个救助性的NPR-2启动子驱动GFP转基因，我们发现NPR-2在多个感觉神经元和中间神经元中表达。基因挽救实验表明，在AIZ中间神经元中表达的NPR-2是MESA避免所必需的。我们还提供了AWB感觉神经元可能作用于RMG和AIZ的上游以检测MESA的证据。我们的结果表明，NPR-2在调节动物行为方面具有重要作用，NPR-1和NPR-2作用于不同的中间神经元，影响秀丽线虫对MESA的回避反应。
Methyl salicylate (MeSa) is a stress hormone released by plants under attack by pathogens or herbivores. MeSa has been shown to attract predatory insects of herbivores and repel pests. The molecules and neurons underlying animal response to MeSa are not known. Here we found that the nematode elegans exhibits a strong avoidance response to MeSa, which requires the activities of two closely related neuropeptide receptors NPR-1 and NPR-2. Molecular analyses suggest that NPR-1 expressed in the RMG inter/motor neurons is required for MeSa avoidance. An NPR-1 ligand FLP-18 is also required. Using a rescuing npr-2 promoter to drive a GFP transgene, we identified that NPR-2 is expressed in multiple sensory and interneurons. Genetic rescue experiments suggest that NPR-2 expressed in the AIZ interneurons is required for MeSa avoidance. We also provide evidence that the AWB sensory neurons might act upstream of RMGs and AIZs to detect MeSa. Our results suggest that NPR-2 has an important role in regulating animal behavior and that NPR-1 and NPR-2 act on distinct interneurons to affect Caenorhabditis elegans avoidance response to MeSa.