Enterococcus faecalis pCF10-encoded surface proteins PrgA, PrgB (aggregation substance) and PrgC contribute to plasmid transfer, biofilm formation and virulence.
Enterococcus faecalis pCF10-encoded surface proteins PrgA, PrgB (aggregation substance) and PrgC contribute to plasmid transfer, biofilm formation and virulence.
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DOI：
10.1111/mmi.12893
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发表时间：
2015-02
影响因子：
3.6
通讯作者：
Christie PJ
中科院分区：
文献类型：
--
作者：
Bhatty M;Cruz MR;Frank KL;Gomez JA;Andrade F;Garsin DA;Dunny GM;Kaplan HB;Christie PJ
粪肠球菌pCF 10转移在prgQ转移操纵子的信息素诱导后以高频率转移。该操纵子编码三种细胞壁锚定蛋白- PrgA，PrgB（聚集物质）和PrgC -以及IV型分泌系统，通过该系统将质粒递送至受体细胞。在这里，我们定义的贡献Prg表面蛋白质质粒转移，生物膜形成，和毒力线虫感染模型。我们报告说，PrgB和细胞外DNA（eDNA）的组合，而不是PrgA或PrgC，需要广泛的细胞聚集和pCF 10转移在野生型频率。除了PrgB和eDNA之外，PrgA的产生对于肠球菌与非生物表面的广泛结合和稳健的生物膜的形成是必要的。然而，尽管PrgB是哺乳动物感染模型中已知的毒力因子，但我们确定了在蠕虫感染模型中有效杀灭所需的是PrgA和PrgC，而不是PrgB。我们认为E.粪肠球菌在进化过程中保留了Prg样表面功能，用于附着、定殖和稳健的生物膜发育。在自然环境中，这些生物膜是多微生物的组成，并构成信号交换，交配对形成和广泛的横向基因转移的最佳环境。
Enterococcus faecalis pCF10 transfers at high frequencies upon pheromone induction of the prgQ transfer operon. This operon codes for three cell-wall-anchored proteins - PrgA, PrgB (aggregation substance), and PrgC - and a type IV secretion system through which the plasmid is delivered to recipient cells. Here, we defined the contributions of the Prg surface proteins to plasmid transfer, biofilm formation, and virulence using the Caenorhabditis elegans infection model. We report that a combination of PrgB and extracellular DNA (eDNA), but not PrgA or PrgC, was required for extensive cellular aggregation and pCF10 transfer at wild-type frequencies. In addition to PrgB and eDNA, production of PrgA was necessary for extensive binding of enterococci to abiotic surfaces and development of robust biofilms. However, although PrgB is a known virulence factor in mammalian infection models, we determined that PrgA and PrgC, but not PrgB, were required for efficient killing in the worm infection model. We propose that the pheromone-responsive, conjugative plasmids of E. faecalis have retained Prg-like surface functions over evolutionary time for attachment, colonization and robust biofilm development. In natural settings, these biofilms are polymicrobial in composition and constitute optimal environments for signal exchange, mating pair formation, and widespread lateral gene transfer.
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影响因子：
4.4
作者：
DUNNY, GM;LEE, LN;LEBLANC, DJ
通讯作者：
LEBLANC, DJ
影响因子：
6.4
作者：
Barnes, Aaron M. T.;Ballering, Katie S.;Dunny, Gary M.
通讯作者：
Dunny, Gary M.
影响因子：
3.2
作者：
CHRISTIE, PJ;KAO, SM;DUNNY, GM
通讯作者：
DUNNY, GM
DOI：
10.1073/pnas.1212256110
发表时间：
2013-04-23
影响因子：
11.1
作者：
Chatterjee, Anushree;Cook, Laura C. C.;Hu, Wei-Shou
通讯作者：
Hu, Wei-Shou
影响因子：
14.8
作者：
Kelley, Lawrence A.;Sternberg, Michael J. E.
通讯作者：
Sternberg, Michael J. E.