Characterization and Pharmacological Efficacy of Silver Nanoparticles Biosynthesized Using the Bark Extract of Garcinia Kola
Characterization and Pharmacological Efficacy of Silver Nanoparticles Biosynthesized Using the Bark Extract of Garcinia Kola
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DOI：
10.1155/2020/2876019
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发表时间：
2020-11-30
影响因子：
3
通讯作者：
Folorunso, Aderonke Similoluwa
中科院分区：
文献类型：
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作者：
Akintelu, Sunday Adewale;Olugbeko, Seyifunmi Charles;Folorunso, Aderonke Similoluwa
影响人类的细菌感染的犯罪危险正在变得无法忍受。本研究旨在探讨利用藤黄树皮提取物生物合成银纳米粒子（AgNPs）对几种细菌的抗菌活性。从尼日利亚翁多州地区Irele镇的“Gbeleju”农场土地获得新鲜的藤黄果树皮。精确地将0.4g预先粉碎的藤黄果树皮浸入20 mL蒸馏水中，并在60-70摄氏度下加热10分钟，得到提取物。利用紫外光谱仪、傅立叶红外光谱仪、透射电子显微镜和X射线能谱仪对纳米粒子进行了表征。然后，将0.2g银纳米颗粒溶解在2 ml水中，得到100 mg/ml的储备溶液，将其进一步稀释，用于通过圆盘扩散法进行抗菌分析。从生物合成的银纳米颗粒的分析表征获得的结果显示球形颗粒形状，通过TEM分析，颗粒尺寸在12.23至27.90 nm的范围内，平均尺寸为20.07 nm。FTIR分析证实了-OH官能团的存在，用于稳定由于在波长3324.52和3344.21 cm（-1）处的宽峰而形成的银纳米颗粒。EDX分析显示碳、氮、氧、铝、钾、铜和银作为存在于纳米颗粒中的元素。生物合成银纳米颗粒的抗菌筛选结果显示出能够阻碍测试细菌生长的抑制潜力。这项研究确定了生物合成的AgNPs作为治疗细菌感染的药物，也是一种有前途的新型抗菌剂的来源。
The delinquent peril of bacterial infections affecting human kind is becoming unbearable. This study was embarked on to investigate the antimicrobial activity of biosynthesized silver nanoparticles (AgNPs) using Garcinia kola bark extract against some bacteria strains. Fresh barks of Garcinia kola were obtained from the "Gbeleju" farm land in Irele town in Ondo state region of Nigeria. Exactly 0.4 g of previously pulverized bark of Garcinia kola was immersed into 20 mL of distilled water and heated at 60-70 degrees C for 10 minutes yielding the extract. The biosynthesized nanoparticle was characterized with UV spectroscope, Fourier infrared spectroscope (FTIR), transmission electron microscope (TEM), and energy dispersive X-Ray analyzer (EDX). Then, 0.2 g of the silver nanoparticles was dissolved in 2 ml of water to yield 100 mg/ml of the stock solution which was further diluted for the antibacterial analysis via the disc diffusion method. The result obtained from the analytical characterization of the biosynthesized silver nanoparticles revealed a spherical particle shape, particle size in the range of 12.23 to 27.90 nm with an average size of 20.07 nm via TEM analysis. The FTIR analysis confirmed the presence of -OH functional group for the stabilization of the silver nanoparticles formed due to the broad peak at wavelength 3324.52 and 3344.21 cm(-1). The EDX analysis revealed carbon, nitrogen, oxygen, aluminum, potassium, copper, and silver as the elements present in the nanoparticles. Results obtained from the antibacterial screening of the biosynthesized AgNPs showed inhibitory potential that are capable of obstructing the growth of the test bacteria. This investigation ascertained the biosynthesized AgNPs as a remedy for curing bacterial infections and also a promising source for novel antibacterial agent.