Synthesis of calcitonin gene-related peptide (CGRP) by rat arterial endothelial cells
Synthesis of calcitonin gene-related peptide (CGRP) by rat arterial endothelial cells
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DOI：
10.14670/hh-16.1073
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发表时间：
2001-10-01
影响因子：
2
通讯作者：
Fujimoto, S
中科院分区：
文献类型：
--
作者：
Doi, Y;Kudo, H;Fujimoto, S
本实验观察了大鼠胸主动脉和股动脉内皮细胞降钙素基因相关肽（CGRP）的蛋白和mRNA表达。光镜免疫细胞化学显示，CGRP的免疫反应优先位于两个血管的内皮细胞。免疫电镜显示CGRP免疫反应性金颗粒优先定位于粗面内质网和韦伯-帕拉德（WP）体在内皮细胞的cispellum。在内皮细胞上也检测到CGRP前体mRNA信号。我们的研究结果是第一次证明，弹性和大肌肉动脉的内皮细胞合成CGRP和存储它，部分，在WP机构，这意味着CGRP可能作为一个内皮源性舒张因子在这些血管。
We investigated the protein and mRNA expression of calcitonin gene-related peptide (CGRP) in endothelial cells of the rat thoracic aorta and femoral artery. Light microscopic immunocytochemistry revealed that immunoreactivity for CGRP was preferentially located in the endothelium of both vessels. Immunoelectron microscopy showed that CGRP-immunoreactive gold particles were preferentially localized on cisterns of the rough endoplasmic reticulum and on the Weibel-Palade (WP) bodies in the endothelial cells. Prepro CGRP mRNA signals were also detected on the endothelium. Our results are the first to demonstrate that endothelial cells of both elastic and large muscular arteries synthesize CGRP and store it, in part, in WP bodies, implying that CGRP may act as an endothelium-derived relaxing factor in these vessels.