Mapping of KIT adjacent sequences on canid autosomes and B chromosomes.
Mapping of KIT adjacent sequences on canid autosomes and B chromosomes.
复制标题
犬科动物常染色体和 B 染色体上 KIT 相邻序列的定位。
DOI：
10.1159/000097424
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发表时间：
2007
影响因子：
1.7
通讯作者：
Graphodatsky,AS
中科院分区：
文献类型：
--
作者：
Yudkin,DV;Trifonov,VA;Kukekova,AV;Vorobieva,NV;Rubtsova,NV;Yang,F;Acland,GM;Ferguson-Smith,MA;Graphodatsky,AS
B染色体通常被认为是核型中最神秘的元素之一（Camacho，2004）。一般认为哺乳动物B染色体不含任何蛋白质编码基因。在犬科动物B染色体上发现的一个保守的KIT基因改变了这一观点。在这里，我们进行了狐狸和浣熊狗的B染色体中KIT周围序列的分析。在犬科动物B染色体上发现了RPL 23 A假基因。在浣熊犬B染色体上发现了KDR基因3′端片段。在这两个物种中，与常染色体片段同源的B特异性片段的大小估计最小为480 kbp。对犬科动物B染色体的起源和进化进行了讨论。
B chromosomes are often considered to be one of the most mysterious elements of karyotypes (Camacho, 2004). It is generally believed that mammalian B chromosomes do not contain any protein coding genes. The discovery of a conserved KIT gene in Canidae B chromosomes has changed this view. Here we performed analysis of sequences surrounding KIT in B chromosomes of the fox and raccoon dog. The presence of the RPL23A pseudogene was shown in canid B chromosomes. The 3′ end fragment of the KDR gene was found in raccoon dog B chromosomes. The size of the B-specific fragment homologous to the autosome fragment was estimated to be a minimum of 480 kbp in both species. The origin and evolution of B chromosomes in Canidae are discussed.