Polychlorinated biphenyl (PCB) alters acid-sensitivity of cultured neurons derived from the medulla oblongata
Polychlorinated biphenyl (PCB) alters acid-sensitivity of cultured neurons derived from the medulla oblongata
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DOI：
10.1016/j.biocel.2005.02.003
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发表时间：
2005-07-01
影响因子：
4
通讯作者：
Koibuchi, N
中科院分区：
文献类型：
--
作者：
Okada, J;Shimokawa, N;Koibuchi, N
多氯联苯（PCBs）是一种环境污染物，可能会导致不良的健康问题。然而，很少有人知道多氯联苯对延髓，调节呼吸的酸敏感神经元的影响。因此，本研究的目的是检查是否PCB改变培养的大鼠延髓神经元的酸敏感性。当细胞外pH值从7.4变化到7.0时，酸敏感神经元显示去极化，这是通过电压敏感的荧光染料测量的。暴露于PCB（Aroclor 1254）在低pH下的去极化幅度降低，并以剂量依赖性方式增加静息膜电位。综上所述，我们的研究结果表明，PCB可能通过改变酸敏感神经元的膜电位，从而影响呼吸的调节来影响酸敏感性。(c)2005爱思唯尔有限公司保留所有权利。
Polychlorinated biphenyls (PCBs) are known as environmental pollutants that may cause adverse health problems. However, little is known about the effects of PCBs on acid-sensitive neurons of the medulla oblongata, which regulate respiration. Therefore, the present study was designed to examine whether PCB alters acid-sensitivity of cultured neurons derived from the rat medulla oblongata. When extracellular pH was shifted from 7.4 to 7.0, acid-sensitive neurons showed depolarization, which was measured by voltage-sensitive fluorescent dye. Exposure to PCB (Aroclor 1254) decreased the amplitude of depolarization in low pH and increased the resting membrane potential in a dose-dependent manner. Taken together, our results indicate that PCB potentially influences acid-sensitivity through alteration of the membrane potential of acid-sensitive neurons, which could affect the regulation of respiration. (c) 2005 Elsevier Ltd. All rights reserved.