Multichannel microchip electrospray mass spectrometry
Multichannel microchip electrospray mass spectrometry
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DOI：
10.1021/ac9607119
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发表时间：
1997-02-01
影响因子：
7.4
通讯作者：
Karger, BL
中科院分区：
文献类型：
--
作者：
Xue, QF;Foret, F;Karger, BL
将微加工的多通道玻璃芯片成功地连接到电喷雾离子化质谱仪（ESI-RIS）上。从每个缓冲液储存器单独施加高电压，用于从每个通道顺序地喷射样品。MS的采样孔接地，发现100-200 nL/min的液体流量对于维持稳定的电喷射是必要的。微芯片质谱实验对肌红蛋白的检测限低于6 × 10 ~（-8）M。在75%甲醇中的样品成功地进行了分析，具有良好的灵敏度，因为是水溶液样品。并行多通道微芯片系统允许在一个芯片中对标准肽和蛋白质的不同样品进行ESI-MS分析。
Microfabricated multiple-channel glass chips were successfully interfaced to an electrospray ionization mass spectrometer (ESI-RIS), The microchip device was fabricated by standard photolithographic, wet chemical etching, and thermal bonding procedures. A high voltage was applied individually from each buffer reservoir for spraying sample sequentially from each channel, With the sampling orifice of the MS grounded, it was found that a liquid flow of 100-200 nL/min was necessary to maintain a stable electrospray., The detection limit of the microchip MS experiment for myoglobin was found to be lower than 6 x 10(-8) M. Samples in 75% methanol were successfully analyzed with good sensitivity, as were aqueous samples. The parallel multiple-channel microchip system allowed ESI-MS analysis of different samples of standard peptides and proteins in one chip.