Comparative analyses of LTRs of the ERV-H family of primate-specific retrovirus-like elements isolated from marmoset, African green monkey, and man
Comparative analyses of LTRs of the ERV-H family of primate-specific retrovirus-like elements isolated from marmoset, African green monkey, and man
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DOI：
10.1006/viro.1997.8590
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发表时间：
1997-07-21
期刊：
影响因子：
3.7
通讯作者：
Johansen, T
中科院分区：
文献类型：
--
作者：
Anderssen, S;Sjottem, E;Johansen, T
我们已经从人18号染色体、非洲绿色猴和绒猴中分离出8个内源性逆转录病毒样元件ERV-H家族的不同长末端重复序列（LTR）。人ERV-H LTR已被分为三种类型，称为I型、Ia型和II型。对45个分离的LTR和60个人ERV-H LTR的比较分析使其能够进一步细分为13个亚型。I型元素在3种植物中均有广泛分布。它们的平均进化年龄（40 MYr），通过一致序列方法估计，表明它们在新旧世界猴子分化时首次在基因组中扩展。一些非常古老的I型序列的出现表明，ERV-H元素可能已经整合，甚至在原猴和灵长类动物分化之前。在两种猴种中均发现了Ia型和II型元素。发现启动子活性I型和Ia型LTR，而II型LTR无活性。启动子活性I型LTR通常含有紧邻TATA盒3'的功能性GC/GT盒，提供Spl家族蛋白的强结合，而高度启动子活性Ia型元件H6含有位于us增强子区域中的协同作用Spl结合位点。(C)北京：科学出版社.
We have isolated 8 different long terminal repeat (LTR) sequences of the ERV-H family of endogenous retrovirus-like elements from human chromosome 18, 9 from African green monkey, and 28 from marmoset. Human ERV-H LTRs have been divided into three types designated Type I, Type la, and Type II. Comparative analyses of the 45 isolated LTRs and 60 human ERV-H LTRs enabled a further subdivision into 13 subtypes. Type I elements were widely distributed in all three species. Their average evolutionary age (40 MYr), estimated by a consensus sequence approach, suggests that they first expanded in the genomes at the time New- and Old World monkeys diverged. The occurence of some very old Type I sequences indicate that ERV-H elements may have integrated even before prosimians and primates diverged. Type la and II elements were found in both monkey species. Promoter active Type I and Type la LTRs were found while Type II LTRs were inactive. Promoter active Type I LTRs generally contained a functional GC/GT box immediately 3' to the TATA box, providing strong binding of Spl family proteins, while the highly promoter active Type la element H6 contained synergistically acting Spl binding sites located in the us enhancer region. (C) 1997 Academic Press.