Effect of dietary supplementation with yeastSaccharomyces cerevisiaeon skin, serum and liver of gilthead seabream (Sparus aurataL)
Effect of dietary supplementation with yeastSaccharomyces cerevisiaeon skin, serum and liver of gilthead seabream (Sparus aurataL)
复制标题
DOI：
10.1111/jfb.14449
复制
发表时间：
2020-08-10
影响因子：
2
通讯作者：
Esteban, Maria Angeles
中科院分区：
文献类型：
--
作者：
Espinosa, Cristobal;Esteban, Maria Angeles
研究了饲料中添加酿酒酵母对黄颡鱼（Sparus aurataL.）的影响。4组重复鱼（n= 6）分别饲喂含有0（对照，不添加酵母）或10 mg / kg热杀（30 min, 60℃）S的商业饲料。酵母。4周后，一半的鱼（2个重复）受伤，继续饲喂相同的饲料。伤后3和7 d分别取血、皮肤黏液、皮肤和肝脏标本。结果表明，在受伤后3天采集的鱼血清中钙浓度显著高于对照鱼，而皮肤粘液中的抗氧化酶在受伤后（3天和7天）都发生了改变。组织学分析显示，喂食对照饲料的鱼在受伤部位出现水肿、炎症迹象和白细胞募集，同时表皮层减少。添加酵母不会改变受伤鱼的生长性能，并有助于维持正常的血清钙浓度。此外，在组织学研究中，与对照组相比，饲喂酵母的动物伤口周围炎症的减少是显而易见的。此外，在受伤鱼的肝脏和皮肤中获得了应激相关基因表达水平的增加。总的来说，酵母补充剂似乎是一种功能性和适当的膳食添加剂，可以改善皮肤恢复，减少伤口造成的压力。
The effect of dietary supplementation withSaccharomyces cerevisiaeon gilthead seabream (Sparus aurataL.) was studied. Four replicates of fish (n= 6) were fed with a commercial diet containing 0 (control, no yeast added) or 10 mg per kilogram of heat-killed (30 min, 60 degrees C)S. cerevisiae. After 4 weeks, half of the fish (two replicates) were injured and continued with the same diet. At 3 and 7 days post-wounding, samples of blood, skin mucus, skin and liver were obtained from each fish. The results showed that calcium concentrations were significantly higher (with respect to control fish) in the serum from fish sampled at 3 days post-wounding, whereas antioxidant enzymes in the skin mucus were altered after wounding (at both 3 and 7 days). Histological analyses revealed oedema, signs of inflammation and white cell recruitment together with a reduction in the epidermis layer in the wounded regions of fish fed control diet. Yeast supplementation did not change growth performance and helped maintain the normal serum calcium concentrations in wounded fish. Furthermore, a reduction in inflammation around wounds in the animals fed yeast with respect to that fed control diet was evident in the histological study. Furthermore, increased levels of stress-related gene expression in liver and skin from wounded fish were obtained. Overall, yeast supplementation seemed to be a functional and appropriate dietary additive to improve skin recovery reducing the stress resulting from wounds.