Genome Sequencing and Characterization of Bacillus velezensis N23 as Biocontrol Agent against Plant Pathogens
Genome Sequencing and Characterization of Bacillus velezensis N23 as Biocontrol Agent against Plant Pathogens
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DOI：
10.3390/microorganisms12020294
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发表时间：
2024-01
期刊：
影响因子：
4.5
通讯作者：
Panlei Yang;Qingchao Zeng;Wenxiao Jiang;Luotao Wang;Jie Zhang;Zhenshuo Wang;Qi Wang;Yan Li
中科院分区：
文献类型：
--
作者：
Panlei Yang;Qingchao Zeng;Wenxiao Jiang;Luotao Wang;Jie Zhang;Zhenshuo Wang;Qi Wang;Yan Li
过度使用化学杀真菌剂对抗真菌病原体对土壤和植物健康产生不利影响，导致环境问题和食品安全。因此，生物防治被认为是环境保护和农业生产中一种环境友好、经济高效的绿色技术。从污染平板上筛选到一株对炭疽病有明显抑制作用的菌株N23。根据16S rRNA基因、gyrA基因和全基因组序列分析，将N23菌株鉴定为Velezensis芽孢杆菌。N23菌株能抑制多种植物病原真菌在固体培养基上的菌丝生长。用菌株N23处理的番茄种子表现出显著高于未处理的种子的发芽水平。此外，菌株N23还能有效地降低辣椒炭疽病的发病面积。基于基因组挖掘和PCR技术，在N23的基因组序列中鉴定了负责抗真菌代谢产物（芬枯草菌素、表面活性素和伊枯草菌素）的基因簇。此外，细菌培养物的甲醇提取物对真菌炭疽菌和灰葡萄孢的生长造成显著抑制。这些结果表明，B。Velezensis N23在农业生产上具有潜在的生防作用，也是一种具有开发价值的抗菌物质来源。
The overuse of chemical fungicides against fungal pathogens adversely affects soil and plant health, resulting in environmental problems and food safety. Therefore, biocontrol is considered as an environmentally friendly and cost-effective green technique in environmental protection and agricultural production. We obtained a bacterial strain N23 from a contaminated plate which showed significant inhibition to anthracnose. The strain N23 was identified as Bacillus velezensis based on 16S rRNA gene, gyrA gene, and whole-genome sequence. The bacterium N23 was able to suppress the mycelial growth of numerous plant pathogenic fungi on solid media. Tomato seeds treated with strain N23 showed significantly higher germination levels than untreated ones. Moreover, strain N23 effectively reduced the lesion area of pepper anthracnose disease in planta. The gene clusters responsible for antifungal metabolites (fengycin, surfactin, and iturin) were identified in the genome sequence of N23 based on genome mining and PCR. Furthermore, methanol extracts of the bacterial culture caused significant inhibition in growth of the fungal Colletotrichum sp. and Botrytis cinerea. These findings suggested that B. velezensis N23 could be a potential biocontrol agent in agricultural production and a source of antimicrobial compounds for further exploitation.