Vitamin A exerts a photoprotective action in skin by absorbing ultraviolet B radiation
Vitamin A exerts a photoprotective action in skin by absorbing ultraviolet B radiation
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DOI：
10.1046/j.1523-1747.2003.12519.x
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发表时间：
2003-11-01
影响因子：
6.5
通讯作者：
Saurat, JH
中科院分区：
文献类型：
--
作者：
Antille, C;Tran, C;Saurat, JH
视黄酯是维生素A的一种储存形式，集中在表皮中，并吸收紫外线辐射，最大吸收波长为325 nm。我们想知道视黄酯的这些吸收特性是否具有生物相关的过滤活性。我们首先使用体外模型来评估棕榈酸视黄酯的光保护特性。然后，我们在将无毛小鼠暴露于1 J/cm（2）紫外线B之前，将棕榈酸视黄酯局部涂在无毛小鼠的背部，并测定紫外线B暴露后2小时表皮DNA中产生的胸腺嘧啶二聚体的水平。最后，我们在人类志愿者的臀部局部涂抹棕榈酸视黄酯或防晒霜，然后将其暴露于四种最低红斑剂量的紫外线B;我们测定了紫外线B暴露后2小时产生的胸腺嘧啶二聚体的水平，并确定了红斑的强度紫外线B后24小时。在体外，棕榈酸视黄酯被证明是有效的商业过滤器辛基甲氧基肉桂酸酯在防止紫外线诱导的荧光或光漂白的荧光标记。棕榈酸视黄酯可明显抑制小鼠表皮胸腺嘧啶二聚体的形成。在人类受试者中，局部使用棕榈酸视黄酯在预防晒伤红斑以及胸腺嘧啶二聚体形成方面与防晒系数为20的防晒霜一样有效。这些结果表明，表皮视黄酯具有生物相关的过滤活性，并建议，除了他们的多形性生物作用，维生素A的新作用，集中在表皮。
Retinyl esters, a storage form of vitamin A, concentrate in the epidermis, and absorb ultraviolet radiation with a maximum at 325 nm. We wondered whether these absorbing properties of retinyl esters might have a biologically relevant filter activity. We first used an in vitro model to assess the photoprotective properties of retinyl palmitate. We then applied topical retinyl palmitate on the back of hairless mice before exposing them to 1 J per cm(2) ultraviolet B, and assayed the levels of thymine dimers produced in epidermal DNA 2 h following ultraviolet B exposure. Finally, we applied topical retinyl palmitate or a sunscreen on the buttocks of human volunteers before exposing them to four minimal erythema doses of ultraviolet B; we assayed the levels of thymine dimers produced 2 h following ultraviolet B exposure, and determined the intensity of erythema 24 h after ultraviolet B. In vitro, retinyl palmitate was shown to be as efficient as the commercial filter octylmethoxycinnamate in preventing ultraviolet-induced fluorescence or photobleaching of fluorescent markers. The formation of thymine dimers in mouse epidermis was significantly inhibited by topical retinyl palmitate. In human subjects, topical retinyl palmitate was as efficient as a sun protection factor 20 sunscreen in preventing sunburn erythema as well as the formation of thymine dimers. These results demonstrate that epidermal retinyl esters have a biologically relevant filter activity and suggest, besides their pleomorphic biologic actions, a new role for vitamin A that concentrates in the epidermis.