Expression of vesicular glutamate transporters in peripheral vestibular structures and vestibular nuclear complex of rat
Expression of vesicular glutamate transporters in peripheral vestibular structures and vestibular nuclear complex of rat
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DOI：
10.1016/j.neuroscience.2010.11.013
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发表时间：
2011-01
期刊：
影响因子：
3.3
通讯作者：
Fu-xing Zhang;Y. Pang;Ming Zhang;Ting Zhang;Yu-Lin Dong;C. Lai;D. Shum;Y. Chan;JL Li;YQ Li
中科院分区：
文献类型：
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作者：
Fu-xing Zhang;Y. Pang;Ming Zhang;Ting Zhang;Yu-Lin Dong;C. Lai;D. Shum;Y. Chan;JL Li;YQ Li
谷氨酸从前庭终器到前庭中枢核复合体（VNC）的传递在头部位置和运动的感觉信息传递中起重要作用。囊泡谷氨酸转运蛋白（VGLUT）的三种亚型被认为是迄今为止最特异的谷氨酸能神经元/细胞的标志物。VGLUT 1和VGLUT 2定位于VNC的轴突终末和前庭初级传入纤维的胞体及其中枢和外周轴突终末，VGLUT 1和VGLUT 3共定位于耳石斑和壶腹嵴的毛细胞。VGLUT 1和VGLUT 2定义了斯卡帕神经元（前庭神经节神经元）的三个子集：共表达VGLUT 1和VGLUT 2或仅表达VGLUT 2的神经元，以及不表达VGLUT 1和VGLUT 2的神经元。此外，位于前庭各亚核的许多神经元均含有VGLUT 2 mRNA的杂交信号，少数VNC神经元（主要分布在前庭内侧核（MVe））显示VGLUT 1 mRNA的标记。单侧神经节切除后，VGLUT 1-免疫反应（ir）和VGLUT 2-ir的不对称性发生在两个VNCs之间，表明含有VGLUT 1和/或VGLUT 2的VNC终末部分是外周起源的。目前的数据表明，组成细胞/神经元沿着前庭通路选择性地应用VGLUT亚型转运谷氨酸到突触囊泡中用于谷氨酸传递。
Glutamate transmission from vestibular end organs to central vestibular nuclear complex (VNC) plays important role in transferring sensory information about head position and movements. Three isoforms of vesicular glutamate transporters (VGLUTs) have been considered so far the most specific markers for glutamatergic neurons/cells. In this study, VGLUT1 and VGLUT2 were immunohistochemically localized to axon terminals in VNC and somata of vestibular primary afferents in association with their central and peripheral axon endings, and VGLUT1 and VGLUT3 were co-localized to hair cells of otolith maculae and cristae ampullaris. VGLUT1 and VGLUT2 defined three subsets of Scarpa's neurons (vestibular ganglionic neurons): those co-expressing VGLUT1 and VGLUT2 or expressing only VGLUT2, and those expressing neither. In addition, many neurons located in all vestibular subnuclei were observed to contain hybridized signals for VGLUT2 mRNA and a few VNC neurons, mostly scattered in medial vestibular nucleus (MVe), displayed VGLUT1 mRNA labelling. Following unilateral ganglionectomy, asymmetries of VGLUT1-immunoreactivity (ir) and VGLUT2-ir occurred between two VNCs, indicating that the VNC terminals containing VGLUT1 and/or VGLUT2 are partly of peripheral origin. The present data indicate that the constituent cells/neurons along the vestibular pathway selectively apply VGLUT isoforms to transport glutamate into synaptic vesicles for glutamate transmission.