Ex vivo characterization of a novel tissue-like cross-linked fibrin-agarose hydrogel for tissue engineering applications
Ex vivo characterization of a novel tissue-like cross-linked fibrin-agarose hydrogel for tissue engineering applications
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DOI：
10.1088/1748-6041/11/5/055004
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发表时间：
2016-10-01
影响因子：
4
通讯作者：
Carriel, Victor
中科院分区：
文献类型：
--
作者：
Campos, Fernando;Bonhome-Espinosa, Ana B.;Carriel, Victor
具有足够的生物力学和结构性能的生物材料的产生仍然是组织工程和再生医学中的一个挑战。早期的研究表明，纳米结构和交联化技术改善了不同生物材料的生物力学和结构特性。目前，未压缩的和纳米结构的纤维蛋白-琼脂糖凝胶(分别为FAH和NFAH)已经成功地应用于组织工程。本研究的目的是探讨使用0.25%和0.5%(v/v)的戊二醛(GA)作为交联剂来改善FAH和NFAH结构和生物力学性能的可能性。对这些非交联型和交联型水凝胶进行了结构、流变学和体外生物相容性分析。结果表明，GA交联剂引起了FAH和NFAH的结构变化，并显著改善了其流变性能。此外，体外生物相容性分析表明细胞在所有条件下都是活的，尽管当使用0.5%GA时，细胞的活力受到更大的影响。我们的研究表明，通过纳米结构或GA交联技术来控制FAH和NFAH的纤维密度和水凝胶孔隙率是可能的。综上所述，以0.25%GA为交联剂的水凝胶具有良好的结构、生物化学和生物学性能，可用于组织工程。
The generation of biomaterials with adequate biomechanical and structural properties remains a challenge in tissue engineering and regenerative medicine. Earlier research has shown that nanostructuration and cross-linking techniques improved the biomechanical and structural properties of different biomaterials. Currently, uncompressed and nanostructured fibrin-agarose hydrogels (FAH and NFAH, respectively) have been used successfully in tissue engineering. The aim of this study was to investigate the possibility of improving the structural and biomechanical properties of FAH and NFAH by using 0.25% and 0.5% (v/v) glutaraldehyde (GA) as a cross-linker. These non-cross-linked and cross-linked hydrogels were subjected to structural, rheological and ex vivo biocompatibility analyses. Our results showed that GA cross-linking induced structural changes and significantly improved the rheological properties of FAH and NFAH. In addition, ex vivo biocompatibility analyses demonstrated viable cells in all conditions, although viability was more compromised when 0.5% GA was used. Our study demonstrates that it is possible to control fiber density and hydrogel porosity of FAH and NFAH by using nanostructuration or GA cross-linking techniques. In conclusion, hydrogels cross-linked with 0.25% GA showed promising structural, biochemical and biological properties for use in tissue engineering.