Organization and expression of the mitochondrial genome in CMS (Moricandia) Brassica juncea: nuclear‐mitochondrial incompatibility results in differential expression of the mitochondrial atpα gene
Organization and expression of the mitochondrial genome in CMS (Moricandia) Brassica juncea: nuclear‐mitochondrial incompatibility results in differential expression of the mitochondrial atpα gene
复制标题
DOI：
10.1111/j.1439-0523.2006.01301.x
复制
发表时间：
2006-12
期刊：
影响因子：
2
通讯作者：
K. Gaikwad;A. Baldev;P. Kirti;T. Mohapatra;S. R. Bhat;V. Chopra;S. Prakash
中科院分区：
文献类型：
--
作者：
K. Gaikwad;A. Baldev;P. Kirti;T. Mohapatra;S. R. Bhat;V. Chopra;S. Prakash
对芥菜型油菜CMS（Moricandia）的线粒体基因组进行了研究。使用9种不同的线粒体特异性基因探针进行Southern分析，发现B之间存在差异。juncea和M. aryensis和CMS系。一个北方配置文件的花蕾和叶片的mt RNA印迹被发现保守的六个基因测试。然而，观察到atpα的组织特异性表达。一个较长的转录本存在于不育系的芽组织中，在育性恢复基因的存在下恢复正常，而其在叶组织中的表达是正常的。从B中克隆了atpα基因。juncea、灯心草M. arvensis和CMS系。限制性酶切图谱显示CMS和B之间的限制性酶切位点高度保守。juncea线在5'和3'端有微小的改变，表明存在几乎完整的M。arvensis atpα基因在含有一个B的不育系中存在。芥菜核本文还对atpα在细胞质雄性不育（Mori）B中的作用进行了讨论。Juncea。
The mitochondrial genome of CMS (Moricandia) Brassica juncea has been characterized. Southern analysis using nine different mt-specific gene probes revealed differences between B. juncea and M. aryensis and the CMS line. A northern profile of flower bud and leaf mt RNA blots was found conserved for six genes tested. However, tissue-specific expression was observed for atpα. A longer transcript was present in the bud tissue of the CMS line, which reverted to normal in the presence of the fertility restorer gene, while its expression was normal in the leaf tissues. The atpα gene was cloned from B. juncea, M. arvensis and the CMS line. Restriction mapping shows a high degree of restriction site conservation between the CMS and B. juncea lines with minor alterations at the 5' and the 3' ends, indicating the presence of an almost intact M. arvensis atpα gene in the CMS line harbouring a B. juncea nucleus. The results are discussed with respect to the potential role of atpα in generating male sterility in CMS (Mori) B. juncea.