Expression of cytoplasmic p16 and anion exchanger 1 is associated with the invasion and absence of lymph metastasis in gastric carcinoma
Expression of cytoplasmic p16 and anion exchanger 1 is associated with the invasion and absence of lymph metastasis in gastric carcinoma
复制标题
胞质p16和阴离子交换剂1的表达与胃癌的侵袭和无淋巴转移有关。
DOI：
10.3892/mmr_00000079
复制
发表时间：
2009-03-01
影响因子：
3.4
通讯作者：
Fu, Guo-Hui
中科院分区：
文献类型：
--
作者：
Liu, Qiang;Song, Ling-Jun;Fu, Guo-Hui
肿瘤抑制因子p16是细胞周期的负调节因子，通常认为在细胞核中起作用。我们最近发现p16蛋白在胃癌细胞的胞浆中表达，并与阴离子交换蛋白1（AE1）同时存在。本研究旨在探讨胃癌组织中p16蛋白表达的意义及其与AE1的关系。应用免疫组化方法检测196例胃癌组织中p16和AE1的表达，其中早期胃癌98例，进展期胃癌98例。统计分析胞浆p16蛋白表达与临床病理特征的关系以及与AE1的关系。p16蛋白在早期胃癌中表达于细胞核，而在进展期胃癌中主要表达于细胞浆。此外，p16的胞浆表达与AE1的表达相关，并且两者都与胃癌中没有淋巴结转移有关。总之，p16蛋白在进展期胃癌中的表达是一个很好的预后指标。p16和AE1共定位预测胃癌无转移胞浆p16和AE1的作用，以及参与胃癌进展的机制，值得进一步研究。
The tumor suppressor p16 is a negative regulator of the cell cycle, commonly believed to act in the nucleus. We recently found that p16 protein is expressed in the cytoplasm of gastric cancer cells, concomitantly with anion exchanger 1 (AE1). The aim of this study was to analyze the significance of cytoplasmic p16 and its relationship to AE1 in the progression of gastric cancer. Expression of p16 and AE1 was examined by immunohistochemical analysis in 196 patients; 98 with early gastric cancer and 98 with advanced gastric cancer. The relationship between cytoplasmic p16 and clinicopathological features, and the relationship between cytoplasmic p16 and AE1, were analyzed statistically. Expression of p16 was observed in the nucleus in early stage gastric cancer, but was located mainly in the cytoplasm in advanced cancer cells. Furthermore, cytoplasmic expression of p16 was correlated with AE1 expression, and both were associated with the absence of lymph metastasis in gastric cancer. In conclusion, cytoplasmic immunoreactivity of p16 appears to be a good prognostic indicator in advanced gastric cancer. Co-localization of p16 and AE1 predicts a lack of metastasis in gastric cancer. The role of cytoplasmic p16 and AE1, and the mechanisms involved in the progression of gastric cancer, warrant further investigation.