Growth rate control of flagellar assembly in Escherichia coli strain RP437
Growth rate control of flagellar assembly in Escherichia coli strain RP437
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DOI：
10.1038/srep41189
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发表时间：
2017-01-24
影响因子：
4.6
通讯作者：
Aldridge, Phillip D.
中科院分区：
文献类型：
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作者：
Sim, Martin;Koirala, Santosh;Aldridge, Phillip D.
鞭毛是一个旋转马达，使细菌能够在液体中游泳并在表面上游动。许多全球调节器控制鞭毛组装响应细胞和环境因素。先前的研究也表明鞭毛的组装受细胞生长速率的影响。然而，尚未描述在受控生长条件下的系统研究。在这里，我们研究了生长速率对鞭毛组装在大肠杆菌中使用稳态恒化培养，我们可以精确地控制细胞的生长速率的影响。我们的研究结果表明，鞭毛丰度与生长速度，其中生长速度更快的细胞产生更多的鞭毛。他们还证明，这种生长速率依赖性控制通过鞭毛主调节因子FlhD（4）C（2）的表达发生。总的来说，我们的研究结果表明，运动是密切耦合到细胞的生长速率。
The flagellum is a rotary motor that enables bacteria to swim in liquids and swarm over surfaces. Numerous global regulators control flagellar assembly in response to cellular and environmental factors. Previous studies have also shown that flagellar assembly is affected by the growth-rate of the cell. However, a systematic study has not yet been described under controlled growth conditions. Here, we investigated the effect of growth rate on flagellar assembly in Escherichia coli using steady-state chemostat cultures where we could precisely control the cell growth-rate. Our results demonstrate that flagellar abundance correlates with growth rate, where faster growing cells produce more flagella. They also demonstrate that this growth-rate dependent control occurs through the expression of the flagellar master regulator, FlhD(4)C(2). Collectively, our results demonstrate that motility is intimately coupled to the growth-rate of the cell.