Overexpression of TRB3 gene in adipose tissue of rats with high fructose-induced metabolic syndrome
Overexpression of TRB3 gene in adipose tissue of rats with high fructose-induced metabolic syndrome
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高果糖代谢综合征大鼠脂肪组织TRB3基因过表达
DOI：
10.1507/endocrj.k08e-049
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发表时间：
2008-08-01
影响因子：
2
通讯作者：
Zhang, Wei
中科院分区：
文献类型：
--
作者：
Bi, Xiu-ping;Tan, Hong-wei;Zhang, Wei
胰岛素抵抗是代谢综合征的病理生理基础。TRB 3已被揭示通过直接与Akt相互作用并阻断其活化而参与肝脏中的胰岛素抵抗。本研究旨在探讨大鼠脂肪组织TRB 3 mRNA表达与代谢综合征的关系。研究分为两组：对照组（Control，n = 12）饲喂标准啮齿动物饲料，实验组（果糖，n = 9）饲喂高果糖饲料。每4周测量一次体重和收缩压。在38周结束时，通过定量实时聚合酶链反应（PCR）测定脂肪组织中tribbles mRNA的水平，并通过Western blot评估Akt/磷酸化Akt表达。结果显示，两组大鼠的脂肪组织中均表达TRB 1 -3 mRNA水平，TRB 1（对照：0.00515，果糖：0.00497）、TRB 2（对照：0.02104，果糖：0.01988）和TRB 3（对照：0.00457，果糖：0.00822）的mRNA水平分别为TRB 1（对照：0.00515，果糖：0.00497）、TRB 2（对照：0.02104，果糖：0.01988）和TRB 3（对照：0.00457，果糖：0.00822）。其中，果糖组大鼠脂肪组织中TRB 3 mRNA表达较对照组增加94%（P < 0.05），且TRB 3 mRNA表达与HOMA-R呈显著正相关（r=0.68，P < 0.05）。Western blot分析显示，果糖组大鼠脂肪组织中磷酸化Akt（Ser-473）的表达明显低于对照组（P < 0.001）。本研究提示TRB 3可能通过抑制脂肪组织中Akt的活化而参与代谢综合征的发生。
Insulin resistance is the physiopathologic foundation of metabolic syndrome. TRB3 has been revealed to be involved in insulin resistance in the liver by interacting directly with Akt and blocking its activation. Our investigation aims at exploring the relationship between metabolic syndrome and TRB3 mRNA expression in adipose tissue of rats. Two groups were Studied as follows: the control group (Control, n = 12) was fed a standard rodent chow, and the experimental group (Fructose n = 9) was fed a high-fructose diet. Body weight-and systolic blood pressure were measured per 4 weeks. At the end of 38 weeks, levels of tribbles mRNAs in adipose tissue were determined by quantitative real-time polymerase chain reaction (PCR), and Akt/phospho-Akt expression was assessed by Western blot. Results show that levels of TRB1-3 mRNAs were expressed in adipose tissue of rats of both groups, and tribbles mRNAs were TRB1 (Control: 0.00515, Fructose: 0.00497), TRB2 (Control: 0.02104, Fructose: 0.01988), and TRB3 (Control: 0.00457, Fructose: 0.00822), respectively. Of the three, TRB3 mRNA alone significantly increased by 94% in adipose tissue of fructose-fed rats compared with those in adipose tissue of the controls (P < 0.05), and there was significant positive correlation between TRB3 mRNA levels and HOMA-R in fructose group (r=0.68, P < 0.05). Western blot analysis showed that phospho-Akt (Ser-473) expression was significantly decreased in adipose tissue of fructose-fed rats compared with controls (P < 0.001). The present study suggests that TRB3 may be involved in metabolic syndrome by inhibiting activation of Akt in adipose tissue.