GENETIC-CONTROL OF THE HEXOSE PHOSPHATE-TRANSPORT SYSTEM OF ESCHERICHIA-COLI - MAPPING OF DELETION AND INSERTION MUTATIONS IN THE UHP REGION
GENETIC-CONTROL OF THE HEXOSE PHOSPHATE-TRANSPORT SYSTEM OF ESCHERICHIA-COLI - MAPPING OF DELETION AND INSERTION MUTATIONS IN THE UHP REGION
复制标题
DOI：
10.1128/jb.155.3.1052-1061.1983
复制
发表时间：
1983-01-01
影响因子：
3.2
通讯作者：
SHATTUCKEIDENS, DM
中科院分区：
文献类型：
--
作者：
KADNER, RJ;SHATTUCKEIDENS, DM
负责积累大量糖磷酸盐的大肠杆菌转运系统由 uhp 区域编码，并由外部而非细胞内的葡萄糖 6-磷酸诱导。为了描绘 uhp 区域的遗传组织，总共收集了 225 个独立点、缺失和转座子 Tn10 插入突变。赋予Uhp-表型的突变是基于它们对磷霉素的抗性以及它们不能使用磷酸糖作为碳源而获得的。 uhp 序列的删除是由于不精确切除位于 uhp 两侧的 Tn10 插入片段而获得的。这些缺失和插入突变点之间的结合杂交可以确定 uhp 等位基因和缺失终点的相对顺序。专业 .lambda。分离携带uhpT-lac操纵子融合体和不同量的相邻uhp材料的转导噬菌体并用作遗传供体。这些杂交的结果证实了在共轭杂交中获得的结果。将突变等位基因的位置与这些等位基因的 Uhp+ 回复体的调控特性进行比较。该比较表明至少存在 3 个基因，其中突变产生 Uhp-表型。作图实验与基因顺序pyreE-gltS-uhpTRA-ilvB一致，其中uhpT编码转运系统，uhpR和uhpA是调节基因，其产物是适当的uhp调节所必需的。
The E. coli transport system responsible for the accumulation of a number of sugar phosphates is encoded by the uhp region and is induced by external, but not intracellular, glucose 6-phosphate. To delineate the genetic organization of the uhp region, a total of 225 independent point, deletion and transposon Tn10 insertion mutations were collected. Mutations conferring the Uhp- phenotype were obtained on the basis of their resistance to fosfomycin and their inability to use sugar phosphates as C source. Deletions of uhp sequences were obtained as a consequence of imprecise excision of Tn10 insertions located on either side of uhp. Conjugal crosses between these deletions and the point of insertion mutations allowed determination of the relative order of the uhp alleles and of the deletion endpoints. Specialized .lambda. transducing phages carrying a uhpT-lac operon fusion and various amounts of adjacent uhp material were isolated and used as genetic donors. Results from these crosses corroborated those obtained in the conjugal crosses. The locations of the mutant alleles were compared with the regulatory properties of Uhp+ revertants of these alleles. This comparison suggested the existence of at least 3 genes in which mutation yields the Uhp- phenotype. Mapping experiments were consistent with the gene order pyreE-gltS-uhpTRA-ilvB, where uhpT encodes the transport system and uhpR and uhpA are regulatory genes whose products are necessary for proper uhp regulation.