Production of Cycloisomaltooligosaccharides from Dextran Using Enzyme Immobilized in Multilayers onto Porous Membranes
Production of Cycloisomaltooligosaccharides from Dextran Using Enzyme Immobilized in Multilayers onto Porous Membranes
复制标题
利用多孔膜多层固定化酶从葡聚糖生产环异麦芽低聚糖
DOI：
10.1021/bp0200245
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发表时间：
2002
影响因子：
2.9
通讯作者：
H. Kawamoto
中科院分区：
文献类型：
--
作者：
H. Kawakita;K. Sugita;Kyoichi Saito;M. Tamada;T. Sugo;H. Kawamoto
采用厚度约1.2 mm、孔径约0.30μm的阴离子交换多孔中空纤维膜作为固定化环异麦芽低聚糖葡聚糖转移酶的载体。CITase通过阴离子交换吸附和随后与转谷氨酰胺酶交联的方法固定在膜上，每克膜的CITase量在3~110 mg之间。酶的多层结合度相当于0.3~9.8。以葡聚糖为底物，以2.0%(w/w)的葡聚糖溶液透过CITase固定的中空纤维膜，以最高的质量产率28%(质量分数)转化为7~9元的环异麦芽寡糖(CI-7、-8和-9)。顺式反应的产率随CITase多层化程度的增加而增加。
Anion‐exchange porous hollow‐fiber membranes with a thickness of about 1.2 mm and a pore size of about 0.30 μm were used as a supporting matrix to immobilize cycloisomaltooligosaccharide glucanotransferase (CITase). CITase was immobilized to the membrane via anion‐exchange adsorption and by subsequent enzymatic cross‐linking with transglutaminase, the amount of which ranged from 3 to 110 mg per gram of the membrane. The degree of enzyme multilayer binding was equivalent to 0.3–9.8. Dextran, as the substrate, was converted into seven‐ to nine‐glucose‐membered cycloisomaltooligosaccharides (CI‐7, ‐8, and ‐9) at a maximum yield of 28% in weight at a space velocity of 10 per hour during the permeation of 2.0% (w/w) dextran solution across the CITase‐immobilized porous hollow‐fiber membrane. The yield of CIs increased with increasing degree of CITase multilayering.
蛋白质与接枝到多孔中空纤维膜上的两性聚合物刷结合
DOI：
--
发表时间：
2007
期刊：
Biotechnology Progress 23
影响因子：
--
作者：
岩撫 暁生;斎藤 恭一
通讯作者：
斎藤 恭一