Recruitment of HIV and its receptors to dendritic cell-T cell junctions
Recruitment of HIV and its receptors to dendritic cell-T cell junctions
复制标题
DOI：
10.1126/science.1084238
复制
发表时间：
2003-05-23
期刊：
影响因子：
56.9
通讯作者：
Hope, TJ
中科院分区：
文献类型：
--
作者：
McDonald, D;Wu, L;Hope, TJ
单核细胞来源的树突状细胞（MDDC）可以有效地结合和转移HIV感染性而自身不被感染。使用活细胞显微镜，我们发现，艾滋病毒被招募到网站的细胞接触MDDC。对MDDC和T细胞之间的缀合物的分析揭示，在缺乏抗原特异性信号传导的情况下，T细胞上的HIV受体CD4、CCR5和CXCR4被募集到界面，而MDDC将HIV浓缩到相同区域。我们提出，树突状细胞和T细胞之间的接触，有利于通过局部集中病毒，受体和辅助受体在感染性突触的形成HIV的传播。
Monocyte-derived dendritic cells (MDDCs) can efficiently bind and transfer HIV infectivity without themselves becoming infected. Using live-cell microscopy, we found that HIV was recruited to sites of cell contact in MDDCs. Analysis of conjugates between MDDCs and T cells revealed that, in the absence of antigen-specific signaling, the HIV receptors CD4, CCR5, and CXCR4 on the T cell were recruited to the interface while the MDDCs concentrated HIV to the same region. We propose that contact between dendritic cells and T cells facilitates transmission of HIV by locally concentrating virus, receptor, and coreceptor during the formation of an infectious synapse.