CHARACTERIZATION OF A 2ND HUMAN PULMONARY SURFACTANT-ASSOCIATED PROTEIN SP-A GENE
CHARACTERIZATION OF A 2ND HUMAN PULMONARY SURFACTANT-ASSOCIATED PROTEIN SP-A GENE
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DOI：
10.1165/ajrcmb/6.4.446
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发表时间：
1992-04-01
影响因子：
6.4
通讯作者：
LOCKER, J
中科院分区：
文献类型：
--
作者：
KATYAL, SL;SINGH, G;LOCKER, J
肺表面活性物质是一种脂蛋白复合物，参与维持肺泡的稳定性。SP-A是26 ~ 38kd的主要表面活性剂相关蛋白。人类SP-A基因（SP-A - I）和两个不同的SP-A cdna， MPSAP 1A和MPSAP 6A，已经报道过。我们已经分离并鉴定了第二个人类SP-A基因（SP-A II），该基因似乎编码与先前描述的MPSAP-1A cDNA对应的mRNA。这两个基因由5个外显子、一个起始一致识别序列TATAAA和一个聚腺苷化信号序列组成。两种基因的显著差异贯穿始终。上游区域、内含子I、外显子III和外显子v的非编码部分差异最大，其他外显子和内含子的编码区差异较小。在成人肺中发现了这两个基因的转录本，使用基因特异性寡核苷酸探针进行Northern blot分析。
Pulmonary surfactant is a lipid-protein complex involved in maintaining alveolar stability. SP-A is the major surfactant-associated protein of 26 to 38 kD. A human SP-A gene (SP-A I) and two distinct SP-A cDNAs, MPSAP 1A and MPSAP 6A, have been reported previously. We have isolated and characterized a second human SP-A gene (SP-A II), which appears to code for the mRNA corresponding to the previously described MPSAP-1A cDNA. Both genes consist of five exons, a consensus recognition sequence for initiation, TATAAA, and a polyadenylation signal sequence. Significant divergence in the two genes is observed throughout. The divergence is highest in the upstream region, intron I, exon III, and noncoding portion of exon V. The coding regions of all other exons and the introns show much lower divergence. Transcripts from both genes were found in adult human lung, using gene-specific oligonucleotide probes in Northern blot analysis.