Basic studies on gene therapy of human malignant melanoma by use of the human interferon β gene entrapped in cationic multilamellar liposomes.: 1.: Morphology and growth rate of six melanoma cell lines used in transfection experiments with the human interferon β gene
Basic studies on gene therapy of human malignant melanoma by use of the human interferon β gene entrapped in cationic multilamellar liposomes.: 1.: Morphology and growth rate of six melanoma cell lines used in transfection experiments with the human interferon β gene
复制标题
DOI：
10.1111/j.1582-4934.2001.tb00175.x
复制
发表时间：
2001-10-01
影响因子：
5.3
通讯作者：
Benga, G
中科院分区：
文献类型：
--
作者：
Benga, G
将六种人恶性黑色素瘤细胞系：A375、A375.2、6361、HMV-1、MM 8.1和WM 115以1 × 10（4）个细胞/ml、2 × 10（4）个细胞/ml或3 × 10（4）个细胞/ml的密度接种于补充有10%胎牛血清和抗生素的RPMI培养基中，在37 ℃、5%CO2的潮湿气氛中。A375细胞也在Dulbecco最小Eagle培养基（DMEM培养基）中生长。通过相差光学显微镜研究其形态。接种后第4天，A375细胞和HMV-1细胞的集落呈椭圆形，细胞呈多角形，并定期相互接触。剩余的细胞分散，更长，并随机接触。6361细胞和MM8.1细胞在达到100%融合之前倾向于形成重叠层。黑色素瘤细胞的生长速度和倍增时间有很大差异。在第1天，A375、6361和HMV-1细胞的倍增时间较短（约6 - 12小时），MM8.1细胞的倍增时间较长（约18小时），A375.2和WM 115细胞的倍增时间非常长（范围在26 - 89小时之间）。细胞的倍增时间作为接种时细胞密度的函数也存在差异。另一方面，除MM8.1细胞外，第2天的倍增时间与第1天相比存在差异。
Six cell lines of human malignant melanoma: A375, A375.2, 6361, HMV-1, MM8.1 and WM115 were seeded at densities of 1x10(4) cells/ml, 2x10(4) cells/ml or 3x10(4) cells/ml of RPMI medium supplemented with 10% fetal calf serum and antibiotics in a humidified atmosfere of 5% CO2 at 37degreesC. A375 cells were also grown in Dulbecco's minimum Eagle's medium (DMEM medium). The morphology was studied by phase contrast light microscopy. At 4 days after seeding the colonies of A375 cells and HMV-1 cells were oval-shaped, the cells were polyhedrical and were making contact with each other regularly. The remaining cells were scattered, more elongated, and made contact randomly. 6361 cells and MM8.1 cells tended to form superposed layers before 100% confluency was achieved. There were great differences in the growth rate and doubling time of melanoma cells. The doubling time in day 1 was short (around 6-12 h) in the case of A375, 6361 and HMV-1 cells, longer (around 18h) in the case of MM8.1 cells and very long (ranging between 26 and 89 h) for A375.2 and WM115 cells. There were also differences in the doubling time of cells as a function of the cell density at seeding. On the other hand, except for MM8.1 cells, there were differences between the doubling time in day 2 compared to day 1.