Synthesis of peptide-oligonucleotide conjugates using a heterobifunctional crosslinker.
Synthesis of peptide-oligonucleotide conjugates using a heterobifunctional crosslinker.
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DOI：
10.1002/0471142700.nc0441s42
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发表时间：
2010-09
影响因子：
--
通讯作者：
Chaput, John C
中科院分区：
文献类型：
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作者：
Williams, Berea A R;Chaput, John C
肽-寡核苷酸偶联物（POC）是由核酸部分与多肽部分共价连接组成的分子嵌合体。POC已被用于从治疗到纳米技术的许多应用中，并且最近作为二价蛋白质亲和试剂的组装中的组合试剂。该单元描述了使用异双功能交联试剂琥珀酰亚胺基-4-（N-马来酰亚胺甲基）环己烷-1-羧酸酯（SMCC）合成和纯化POC分子，该试剂使胺修饰的寡核苷酸能够共价连接到半胱氨酸修饰的多肽。该基于溶液的方案由两步合成和随后的单一纯化步骤组成。
Peptide-oligonucleotide conjugates (POCs) are molecular chimeras composed of a nucleic acid moiety covalently attached to a polypeptide moiety. POCs have been used in numerous applications from therapeutics to nanotechnology, and most recently as combinatorial agents in the assembly of bivalent protein affinity reagents. This unit describes the synthesis and purification of POC molecules using the heterobifunctional crosslinking reagent succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC), which enables amine-modified oligonucleotides to become covalently linked to cysteine-modified polypeptides. This solution-based protocol consists of a two-step synthesis followed by a single purification step.