Activated platelets induce monocyte chemotactic protein-1 secretion and surface expression of intercellular adhesion molecule-1 on endothelial cells
Activated platelets induce monocyte chemotactic protein-1 secretion and surface expression of intercellular adhesion molecule-1 on endothelial cells
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DOI：
10.1161/01.cir.98.12.1164
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发表时间：
1998-09-22
期刊：
影响因子：
37.8
通讯作者：
Brand, K
中科院分区：
文献类型：
--
作者：
Gawaz, M;Neumann, FJ;Brand, K
血小板/内皮细胞相互作用在炎症和动脉粥样硬化的病理生理学中起着重要作用。血小板对单核细胞趋化蛋白-1（MCP-1）分泌和内皮细胞表面细胞间粘附分子-1（ICAM-1）表达的作用已被评估。方法和结果-人脐静脉内皮细胞单层与未刺激或ADP活化的血小板孵育6小时，并通过ELISA和流式细胞术测定MCP-1分泌和ICAM-1表面表达，分别ADP活化血小板的MCP-L分泌和ICAM-1表达均较未活化血小板显著增加（P
Background-Platelet/endothelium interaction plays an important role in the pathophysiology of inflammation and atherosclerosis. The role of platelets for monocyte chemotactic protein-1 (MCP-1) secretion and surface expression of intercellular adhesion molecule-1 (ICAM-1) on endothelial cells has been assessed.Methods and Results-Monolayers of human umbilical vein endothelial cells were incubated with nonstimulated or ADP-activated platelets for 6 hours, and secretion of MCP-1 and surface expression of ICAM-1 were determined by ELISA and flow cytometry, respectively. In the presence of ADP-activated platelets, both MCP-L secretion and ICAM-1 surface expression were significantly increased compared with nonstimulated platelets (P