Danhong Promotes Angiogenesis in Diabetic Mice after Critical Limb Ischemia by Activation of CSE-H 2 S-VEGF Axis.
Danhong Promotes Angiogenesis in Diabetic Mice after Critical Limb Ischemia by Activation of CSE-H 2 S-VEGF Axis.
复制标题
丹红通过激活 CSE-H2S-VEGF 轴促进糖尿病小鼠严重肢体缺血后的血管生成
DOI：
10.1155/2015/276263
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Liang C
中科院分区：
文献类型：
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作者：
Wu F;He Z;Ding R;Huang Z;Jiang Q;Cui H;Lin Y;Huang S;Dai X;Zhang J;Wu Z;Liang C
目的：探讨丹红注射液对糖尿病下肢缺血小鼠血管生成的影响及其机制。30只糖尿病后肢缺血模型小鼠和10只正常小鼠，腹腔注射（i. p.）将20只糖尿病后肢缺血模型小鼠随机分为生理盐水对照组（n = 10）和DH腹腔注射组（2 mL/kg体重，连续7天）。分别在干预期间和安乐死后评价肢体灌注恢复和股动脉血硫化氢（H2S）和血管再生以及下肢血管内皮生长因子（VEGF）/胱硫醚γ-裂解酶（CSE）表达。DH腹腔注射增加缺血肢体灌注，促进侧支循环生成，而不降低血糖水平。局部CSE-H2S-VEGF表达增加有助于DH注射的有益作用。结论：DH注射液可能通过激活局部CSE-H2S-VEGF轴参与了糖尿病下肢缺血模型小鼠的促血管生成作用，为糖尿病下肢缺血患者的治疗提供了一种新的途径。
The aim of this paper is to investigate effect and mechanism of Danhong injection (DH) on angiogenesis in the diabetic hind limb ischemia mouse model. Thirty diabetic hind limb ischemic model mice and ten normal mice, established by intraperitoneal (i.p.) injection of streptozotocin (STZ) or PBS and ligation/excision of femoral artery, and then twenty diabetic hind limb ischemic model mice of all were evenly randomized to saline (control, n = 10) and DH i.p. injection (2 mL/kg weight for 7 days, n = 10) groups. Limb perfusion recovery and femoral blood hydrogen sulfide (H2S) and vessel regeneration and lower limb vascular endothelial growth factor (VEGF)/cystathionine γ-lyase (CSE) expression were evaluated during intervention and after euthanasia, respectively. DH i.p. increased ischemic limb perfusion and promoted collateral circulation generation without decreasing blood glucose level. Increased local CSE-H2S-VEGF expression contributed to beneficial effects of DH injection. In conclusion, activation of local CSE-H2S-VEGF axis might participate in proangiogenesis effects of DH injection in diabetic hind limb ischemia model mice, suggesting a potential therapy for diabetic patients with critical limb ischemia.