Isolation and Characterization of Laminin-10/11 Secreted by Human Lung Carcinoma Cells
Isolation and Characterization of Laminin-10/11 Secreted by Human Lung Carcinoma Cells
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人肺癌细胞分泌的 Laminin-10/11 的分离和表征
DOI：
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发表时间：
1998
影响因子：
4.8
通讯作者：
K. Sekiguchi
中科院分区：
文献类型：
--
作者：
Y. Kikkawa;N. Sanzen;K. Sekiguchi
层粘连蛋白α5链是一种新发现的层粘连蛋白亚基，由层粘连蛋白-10 （α5β1γ1）和-11 （α5β2γ1）组成。在肺腺癌细胞系A549中，α5链的表达量较高，而其他α链的表达量未检测到。用抗层粘连蛋白单克隆抗体4C7从A549细胞的条件培养基中纯化了含有α5链的层粘连蛋白变体，该抗体最近被证明可以识别层粘连蛋白α5链(Tiger, c - f。香槟，m.f。佩德罗萨-多默洛夫，F.，桑内尔，L.-E.。（1997） J.生物学。化学，272,28590-28595)。纯化的层粘连蛋白变体由三个分子质量分别为350、220和210 kDa的链组成。350-kDa链被另一种在免疫印迹上能够识别变性α5链的抗α5链单克隆抗体特异性识别，而210-kDa链被抗γ - 1链抗体特异性识别。纯化的含α5链的层粘连蛋白变体（以下简称层粘连蛋白10/11）在介导A549细胞对基质的粘附方面表现出较高的活性，其效力与层粘连蛋白5相当，显著高于层粘连蛋白1、层粘连蛋白2/4和纤连蛋白。针对整合素α3或β1亚基的抗整合素抗体可特异性抑制层粘连蛋白-10/11对基底的粘附，而针对α2或α6亚基的抗整合素抗体则不能，说明层粘连蛋白-10/11可被整合素α3β1特异性识别。鉴于laminin-10/11广泛分布于各种组织类型的基底膜，且整合素α3β1在大多数上皮细胞中均以表达为主，因此laminin-10/11与整合素α3β1的特异性相互作用可能在体内通过基底膜调控上皮细胞的增殖和分化中发挥重要作用。
A panel of human tumor cell lines was screened for selective expression of laminin α5 chain, a newly identified laminin subunit comprising laminin-10 (α5β1γ1) and -11 (α5β2γ1). The lung adenocarcinoma cell line A549 was found to express the α5 chain at relatively high levels but no detectable amounts of other α chains. The laminin variants containing α5 chain were purified from the conditioned medium of A549 cells by immunoaffinity chromatography using the anti-laminin monoclonal antibody 4C7 which was shown recently to recognize the laminin α5 chain (Tiger, C.-F., Champliaud, M.-F., Pedrosa-Domellof, F., Thornell, L.-E., Ekblom, P., and Gullberg, D. (1997) J. Biol. Chem. 272, 28590–28595). The purified laminin variants consisted of three chains with molecular masses of 350, 220, and 210 kDa. The 350-kDa chain was specifically recognized by another anti-α5 chain monoclonal antibody capable of recognizing denatured α5 chain on immunoblots, whereas the 210-kDa chain was recognized by an anti-γ1 chain antibody. The purified α5 chain-containing laminin variants (hereafter referred to as laminin-10/11) were highly active in mediating adhesion of A549 cells to the substratum with potency as high as that of laminin-5 and significantly higher than those of laminin-1, laminin-2/4, or fibronectin. Adhesion to substrata coated with laminin-10/11 was specifically inhibited by anti-integrin antibodies directed against the integrin α3 or β1 subunit but not by those against α2 or α6 subunit, indicating that laminin-10/11 is specifically recognized by integrin α3β1. Given the wide distribution of laminin-10/11 in the basement membrane of various tissue types and dominant expression of integrin α3β1 in most epithelial cells, specific interaction of laminin-10/11 with integrin α3β1 may play an important role in in vivo regulation of proliferation and differentiation of epithelial cells through the basement membrane.
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通过有限的蛋白水解从胎盘中分离出几乎完整的、具有生物活性的人层粘连蛋白。
DOI：
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发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wewer,U;Albrechtsen,R;Manthorpe,M;Varon,S;Engvall,E;Ruoslahti,E
通讯作者：
Ruoslahti,E
DOI：
10.1073/pnas.87.9.3264
发表时间：
1990-05-01
影响因子：
11.1
作者：
EHRIG, K;LEIVO, I;ENGVALL, E
通讯作者：
ENGVALL, E
影响因子：
4
作者：
Scott E. Baker;S. Hopkinson;M. Fitchmun;G. Andreason;Francine Frasier;George;Plopper;Vito Quaranta;Jonathan C. R. Jones
通讯作者：
Scott E. Baker;S. Hopkinson;M. Fitchmun;G. Andreason;Francine Frasier;George;Plopper;Vito Quaranta;Jonathan C. R. Jones
人血浆纤连蛋白的结构域。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Sekiguchi,K;Hakomori,S
通讯作者：
Hakomori,S
锚定丝蛋白加里宁是作为高分子量前体合成和分泌的。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Marinkovich,MP;Lunstrum,GP;Burgeson,RE
通讯作者：
Burgeson,RE