Improved fluorescent compounds for tracing cell lineage.
Improved fluorescent compounds for tracing cell lineage.
复制标题
改进的荧光化合物用于追踪细胞谱系。
DOI：
10.1016/0012-1606(85)90476-2
复制
发表时间：
1985
影响因子：
2.7
通讯作者：
Braun,J
中科院分区：
文献类型：
--
作者：
Gimlich,RL;Braun,J
本文介绍了几种新型荧光细胞系示踪剂的合成方法。这些是平均分子量约为11 × 103的荧光葡聚糖，每个葡聚糖链上有一个或多个共价偶联的荧光团分子。这些荧光右旋糖酐比市售产品更亮，可以使用气压注射或离子电泳进行微注射。它们持续时间长，在胚胎细胞的细胞质中分布均匀，清晰地显示出非常精细的细胞延伸，如纤毛、轴突和柄足。还描述了一种通过醛处理将游离氨基共价连接到荧光葡聚糖以使示踪剂与细胞成分可共固定的方法。荧光右旋氨基示踪剂允许胚胎细胞系研究固定，渗透，或切片胚胎。
In this note simple methods for the synthesis of several new fluorescent cell lineage tracers are described. These are fluorescent dextrans with average molecular weights of approximately 11 × 103, and with one or more fluorophore molecules covalently coupled to each dextran chain. These fluorescent dextrans are brighter than commercially obtainable products, and can be microinjected using either air-pressure injection or iontophoresis. They are long-lasting and have a uniform distribution in the cytoplasm of embryonic cells, clearly revealing very fine cell extensions such as cilia, axons, and filipodia. A method is also described for covalently attaching free amino groups to fluorescent dextran to make the tracers cofixable with cellular constituents by aldehyde treatment. Fluorescent dextran-amine tracers allow embryonic cell lineages to be studied in fixed, permeabilized, or sectioned embryos.